OPTICAL MAPPING COMPONENT

大的插入克隆如细菌人工 染色体（BAC）提供了相对明确的标记， 可解释和便于首先形成重叠群，其次， 序列读取比对。我们开发了一种新的方法来限制 BAC的映射可以很容易地自动化， 采样并生成高分辨率有序限制性图谱。的目的 项目的这一部分是构建序列就绪，有序的限制 图18染色体BAC克隆衍生自一个连续的36 Mb区域， 人类18号染色体。我们将使用这些地图，首先创建一个高 分辨率最小平铺路径从数据产生的博士麦克弗森使用 限制性片段指纹分析，其次，构建支架， 可靠地组装由D.麦康比·安 有序的限制性图谱支架将显著减少 需要进行序列整理。此外，我们的地图将确定 含有重复序列的区域给序列组装带来困难 并提供一种检验序列组装的方法。我们将为此提供便利 随着算法的构建和软件的开发， 调整地图以加强重叠群的形成，其次， 验证序列组装。这将是特别有价值的染色体 这些区域很难组装。

Restriction maps of large insert clones such as bacterial artificial chromosomes (BAC) provide relatively unambiguous markers that are easily interpretable and facilitate firstly, contig formation and secondly, sequence read alignment. We have developed a new approach to restriction mapping of BACs that can be readily automated, work with small amounts of sample and generate high resolution ordered restriction maps. The aim of this part of the project is to construct sequence ready, ordered restrict maps of chromosome 18 BAC clones derived from a contiguous 36 Mb region of human chromosome 18. We will use these maps, firstly to create a high resolution minimum tiling path from data generated by Dr. McPherson using restriction fragment fingerprinting and secondly, to crete a scaffold to reliably assemble islands of sequence data generated by D. McCombie An ordered restriction map scaffold will significantly reduce the effort required for sequence finishing. Furthermore, our maps will identify regions containing repeats which pose difficulties in sequence assembly and provide a means to verify sequence assembly. We will facilitate this with the construction of algorithms and development of software firstly for the alignment of maps to strengthen contig formation and secondly to verify sequence assembly. This will be of particular value in chromosomal regions which are difficult to assemble.