CORE--ELECTROPHYSIOLOGY

核心--电生理学

• 批准号: 6108852
• 负责人: ROBERT D SKINNER
• 金额: $ 11.03万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6108852
• 关键词: biomedical facility; electromyography; electrophysiology; exercise; motor neurons; neuromuscular function; sectioning; spinal cord injury; spinal reflex

The purpose of the Electrophysiology Core will be to provide centralized electrophysiological services to each of the Components of the Program Project. Three types of specialized measures will be carried out: H-reflex measures in vivo in Components I, II, III and IV, muscle force and fatigability testing in situ in Components I and II, and intracellular labeling in vitro in Components III and IV. The Core Facility will consist of two contiguous rooms (one 350 sq. ft. and one 175 sq. ft.) containing the cat and rat exercise/treadmill set-ups in the larger, divided room, and a slice recording set-up In the smaller, undivided room. The contiguity will allow certain common pieces of equipment to be shared between rooms, representing significant savings. For in vivo H-reflex testing, amplifiers (electromyograms) and stimulators will be required, while in situ recording of muscle force and fatigability will require the addition of force transducers and a spinal stereotaxic instrument and surgical table. Computer data analysis (Superscope) and storage (Neurodata) will be shared across measures, while the in vitro recordings will require an intracellular amplifier, dissecting microscope, vibration damping air table and electrode carrier. Some of this equipment is on hand and only about one third of the equipment required for this triple-duty Facility is being requested. The centralized recording of different measures will allow standardization of results across Components, permitting comparisons across paradigms and species.

电生理学核心的目的是提供 集中的电生理服务， 计划项目的组成部分。三种专业 将进行的措施：H-反射措施，在体内， 第一、二、三、四部分：肌肉力量和疲劳性 在组分I和II中原位测试，以及细胞内标记 在组分III和IV中的体外。核心设施将包括 两个相邻的房间（一个350平方米，英尺一个175平方米英尺）。 包含猫和大鼠运动/跑步机设置， 更大的，分开的房间，和一个切片记录设置在较小的， 不分开的房间这种连续性将允许某些共同的片段 各房间之间共用的设备， 积蓄对于体内H反射测试，放大器 （肌电图）和刺激器将需要，而在原位 肌肉力量和疲劳的记录将需要 增加力传感器和脊柱立体定位仪 和手术台计算机数据分析（Superscope）和 存储（神经数据）将在度量之间共享，而 体外记录将需要细胞内放大器， 解剖显微镜、减振空气台和电极 载体其中一些设备是手头上，只有大约一个 该三重任务设施所需设备的三分之一是 被要求。不同措施的集中记录 将允许跨组件的结果标准化， 允许跨范例和物种的比较。