RELOCALIZATION OF CELLULAR PKR DURING MENGO VIRUS INFECTION

我们感兴趣的是细胞对病毒感染的反应 这与宿主致病机制有何关联。作为我们的模型，我们研究 Mengo病毒感染小鼠巨噬细胞。蒙戈病毒感染是 蒙古族病毒感染的致病机制研究 老鼠是很好的特征。在蒙戈病毒感染期间，一个细胞 前哨蛋白，蛋白激酶R(PKR)，从一个 胞质均匀分布于核周。PKR有 被认为在许多细胞反应中起重要作用 其他病毒感染，包括艾滋病毒-1、腺病毒和牛痘 病毒。因此，PKR可能在细胞反应中具有普遍作用。 病毒感染。为了了解蒙戈病毒感染是如何引发 PKR在感染细胞中的重新定位及其在 细胞对病毒感染的反应，我们已经创建了一个小组 减毒的芒果病毒。我们希望使用间接免疫荧光 和共聚焦显微镜来确定这些病毒是否在 在感染细胞中诱导PKR重新定位的能力。

We are interested in the cellular response to viral infection and how that correlates to host pathogenesis. As our model, we study mengo virus infection of murine macrophages. Mengo virus infection is useful to study in that the pathogenesis of mengo virus infection in mice is well characterized. During mengo virus infection, a cellular sentinel protein, protein kinase R (PKR), is relocalized from a uniform cytoplasmic distribution to a perinuclear locale. PKR has been implicated as being important in the cellular response to many other virus infections including, HIV-1, adenovirus, and vaccinia virus. So, PKR may have a universal role in the cellular response to viral infections. To understand how mengo virus infection elicits a relocalization of PKR in infected cells and how this is important in the cellular response to viral infection, we have created a panel of attenuated mengo viruses. We wish to use indirect immunofluorescence and confocal microscopy to determine if these viruses are impaired in the ability to elicit a relocalization of PKR in infected cells.