RELOCALIZATION OF CELLULAR PKR DURING MENGO VIRUS INFECTION

Mengo 病毒感染期间细胞 PKR 的重新定位

• 批准号: 6117330
• 负责人: ANN C. PALMENBERG
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117330
• 关键词: animal tissue; bioimaging /biomedical imaging; biomedical resource; biotechnology; infection; microscopy; nucleic acids; proteins; vaccines; virus

We are interested in the cellular response to viral infection and how that correlates to host pathogenesis. As our model, we study mengo virus infection of murine macrophages. Mengo virus infection is useful to study in that the pathogenesis of mengo virus infection in mice is well characterized. During mengo virus infection, a cellular sentinel protein, protein kinase R (PKR), is relocalized from a uniform cytoplasmic distribution to a perinuclear locale. PKR has been implicated as being important in the cellular response to many other virus infections including, HIV-1, adenovirus, and vaccinia virus. So, PKR may have a universal role in the cellular response to viral infections. To understand how mengo virus infection elicits a relocalization of PKR in infected cells and how this is important in the cellular response to viral infection, we have created a panel of attenuated mengo viruses. We wish to use indirect immunofluorescence and confocal microscopy to determine if these viruses are impaired in the ability to elicit a relocalization of PKR in infected cells.

我们感兴趣的是细胞对病毒感染的反应， 与宿主发病机制的关系 作为我们的模特， 小鼠巨噬细胞的孟戈病毒感染。 门戈病毒感染是 对研究门戈病毒感染的发病机制有一定的意义。 小鼠被很好地表征。 在孟戈病毒感染期间， 哨兵蛋白，蛋白激酶R（PKR），是从一个蛋白质的位置重新定位。 均匀的细胞质分布到核周区域。 PKR拥有 被认为是重要的细胞反应， 其他病毒感染，包括HIV-1、腺病毒和牛痘 病毒 因此，PKR可能在细胞反应中具有普遍作用， 病毒感染 了解门戈病毒如何感染埃博拉病毒 PKR在感染细胞中的重新定位以及这在 细胞对病毒感染的反应，我们已经创建了一个小组， 减毒门戈病毒。 我们希望用间接免疫荧光法 和共聚焦显微镜，以确定这些病毒是否受损， 诱导PKR在感染细胞中重新定位的能力。