VISUALIZATION OF VIRUS INFECTED CELLS

病毒感染细胞的可视化

• 批准号: 6117320
• 负责人: ANN C. PALMENBERG
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117320
• 关键词: bioimaging /biomedical imaging; biomedical resource; biotechnology; human tissue; infection; microscopy; nucleic acids; proteins; vaccines; virus

We have developed a series of attenuated viral vaccine vectors, based on murin Mengovirus, and adapted these vectors to carry large sequences from heterologous proteins. We need to know how efficiently the heterologous proteins are expressed during HeLa cell infection. We intend to infect cells on microscope slides, fix them, then stain them with fluorescent antibodies that can be detected (and recorded) by confocal microscopy. Alternatively, the heterologous protein itself (GFP), might be detected under the scope, using the appropriate wavelength or filters.

我们已经开发了一系列减毒病毒疫苗载体， 基于小鼠孟加拉病毒，并对这些载体进行改造以携带 来自异源蛋白质的序列。我们需要知道有多有效 异源蛋白在HeLa细胞感染过程中表达。 我们打算感染显微镜载玻片上的细胞，修复它们，然后染色 它们带有可被检测(和记录)的荧光抗体 通过共聚焦显微镜。或者，异源蛋白质 本身(GFP)，可以在范围内检测，使用适当的 波长或滤光片。