CALIBER OF ALVEOLAR SEPTAL MICROVESSELS IN ZONE I

我们将测量肺泡间隔的功能口径 分离的大鼠肺中的微血管，已经准备好 肺充气压超过灌注压(1区)。这 导致微血管几乎被压缩到崩溃的程度。 测量将通过灌流分离的大鼠肺进行， 特定直径的荧光乳胶粒(0.010-10.0 微米)。然后肺将被冷冻，通过冷冻来固定 替换和嵌入。切片将使用激光进行检查 共聚焦荧光显微镜。从图像上看，乳胶颗粒 密度将以单个牙槽间隔为单位进行测量。数据将是 比较了不同颗粒直径的肺和肺 用于计算功能性间隔微血管合胞体 在肺灌流的情况下的口径。vbl.使用 这是一种新开发的方法，我们计划进行三维重建 静脉复苏和静脉复苏的血流灌注特性 大鼠离体肺的血红蛋白溶液。它是医学性的 重要的是要遵循这些途径，看看是否有灌流在 毛细管水平。如果这些溶液能在高压下进入毛细血管 肺的充气压力，它作为氧气的携带者会很有用 用于治疗低血容量性休克患者的药物。

We will measure the functional caliber of alveolar septal microvessels in isolated rat lungs which have been prepared so that lung inflation pressure exceeds perfusion pressure (zone 1). This causes the microvessels to compress nearly to the point of collapse. The measurements will be made by perfusion isolated rat lungs with fluorescent latex particles of specific diameters (0.010- 10.0 micrometers). The lungs will then be frozen, fixed by freeze substitution and embedded. Sections will be examined using a laser confocal fluorescence microscope. From the images, latex particle density will be measured in individual alveolar septa. Data will be compared from lungs perfused with different particle diameters and used to calculate the functional septal microvascular syncytial caliber under the conditions in which the lungs were perfused. Using a newly developed method, we plan to reconstruct three dimensionally the perfusion characteristics of intravenous ruscitation and hemoglobin solutions in isolated rat lungs. It is of medical importance to follow these pathways to see if perfusion exists at the capillary level. If these solutions can enter capillaries under high lung inflation pressures, it will be very useful as an oxygen carrying agent in treating hypovolemic shock patients.