CALIBER OF ALVEOLAR SEPTAL MICROVESSELS IN ZONE I

I区肺泡间隔微血管的口径

• 批准号: 6278512
• 负责人: Robert L Conhaim
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6278512
• 关键词: biomedical resource; gas; microscopy; respiratory system

We will measure the functional caliber of alveolar septal microvessels in isolated rat lungs which have been prepared so that lung inflation pressure exceeds perfusion pressure (zone 1). This causes the microvessels to compress nearly to the point of collapse. The measurements will be made by perfusion isolated rat lungs with fluorescent latex particles of specific diameters (0.010- 10.0 micrometers). The lungs will then be frozen, fixed by freeze substitution and embedded. Sections will be examined using a laser confocal fluorescence microscope. From the images, latex particle density will be measured in individual alveolar septa. Data will be compared from lungs perfused with different particle diameters and used to calculate the functional septal microvascular syncytial caliber under the conditions in which the lungs were perfused. Using a newly developed method, we plan to reconstruct three dimensionally the perfusion characteristics of intravenous ruscitation and hemoglobin solutions in isolated rat lungs. It is of medical importance to follow these pathways to see if perfusion exists at the capillary level. If these solutions can enter capillaries under high lung inflation pressures, it will be very useful as an oxygen carrying agent in treating hypovolemic shock patients.

测量肺泡间隔功能口径 已经制备的离体大鼠肺中的微血管， 肺充气压力超过灌注压力（区域1）。 这 导致微血管压缩到几乎崩溃的程度。 将通过灌注分离的大鼠肺进行测量， 荧光乳胶颗粒的比直径（0.010- 10.0 微米）。 然后将肺冷冻，通过冷冻固定 替代和嵌入。 将使用激光检查切片 共聚焦荧光显微镜 从照片上看乳胶颗粒 将在单个肺泡隔中测量密度。 数据将 与灌注不同颗粒直径的肺进行比较， 用于计算功能性间隔微血管合胞体 在肺灌注的条件下的口径。 使用 一种新开发的方法，我们计划三维重建 静脉输液的灌注特点， 血红蛋白溶液中分离的大鼠肺。 它是医学 重要的是要遵循这些途径，看看是否存在灌注在 毛细管水平 如果这些溶液可以在高浓度下进入毛细血管， 肺部充气压力，它作为氧气运输非常有用 治疗低血容量性休克患者。