VISUALIZATION OF CHLOROPLAST 3 DIMENSIONAL ARCHITECTURE BY SEM

通过 SEM 可视化叶绿体 3 维结构

• 批准号: 6278513
• 负责人: ROBERT J WISE
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6278513
• 关键词: biomedical resource; microscopy; plants

We are studying the architecture of chloroplasts. Photosynthesis contains two components "light reactions and "dark " reactions. The light reactions occur on a 3-D membrane network (thykaloids) in chloroplasts and involve linear electron transport through several protein complexes, most notably photosystems II (PSH) and I (PSI). These two protein complexes are found in distinct regions of the thylakoid. Therefore, electrons must be shuttled on mobile, diffusible electron carriers plastouinonone (PQ) and plasotcyanin (PC). The diffusion rates of PC and PQ are known but not the average diffasional distance because the thykaloid 3-D structure is unknown. Without a value for average diffusional distance, theorists are stymied in their desire to mathematically model photosynthetic electron transfer rates. SEM images will be used to derive values for the average diffusional distance between the two photosystems that will then be used to test theoretical models.

我们正在研究叶绿体的结构。光合作用 包含两个组件“光反应”和“暗”反应。 光反应发生在3-D膜网络(类囊体)上 叶绿体和涉及线性电子传递的几个 蛋白质复合体，最著名的是光系统II(PSH)和I(PSI)。 这两种蛋白质复合体位于不同的区域 类囊体。因此，电子必须在移动设备上穿梭， 可扩散电子载体植物红素(PQ)和血浆蓝蛋白 (PC)。PC和PQ的扩散速率是已知的，但不是平均值 扩散距离，因为类囊体的三维结构未知。 没有平均扩散距离的值，理论家们 阻碍了他们对光合作用进行数学建模的愿望 电子转移率。将使用扫描电子显微镜图像来推导以下值 两个光系统之间的平均漫射距离 然后将被用来测试理论模型。