VISUALIZATION OF CHLOROPLAST 3 DIMENSIONAL ARCHITECTURE BY SEM

通过 SEM 可视化叶绿体 3 维结构

• 批准号: 6117318
• 负责人: ROBERT J WISE
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117318
• 关键词: bioimaging /biomedical imaging; biomedical resource; microscopy; plants

We are studying the architecture of chloroplasts. Photosynthesis contains two components "light reactions and "dark " reactions. The light reactions occur on a 3-D membrane network (thykaloids) in chloroplasts and involve linear electron transport through several protein complexes, most notably photosystems II (PSH) and I (PSI). These two protein complexes are found in distinct regions of the thylakoid. Therefore, electrons must be shuttled on mobile, diffusible electron carriers plastouinonone (PQ) and plasotcyanin (PC). The diffusion rates of PC and PQ are known but not the average diffasional distance because the thykaloid 3-D structure is unknown. Without a value for average diffusional distance, theorists are stymied in their desire to mathematically model photosynthetic electron transfer rates. SEM images will be used to derive values for the average diffusional distance between the two photosystems that will then be used to test theoretical models.

我们正在研究叶绿体的结构。光合作用