RECOMBINANT DNA STUDIES OF RAPID/SLOW N-ACETYLTRANSFERASES

快速/慢速 N-乙酰转移酶的重组 DNA 研究

• 批准号: 6274584
• 负责人: WENDELL W WEBER
• 金额: $ 2.15万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6274584
• 关键词: acyltransferase; animal genetic material tag; gene expression; human genetic material tag; laboratory mouse; nucleic acid probes; nucleic acid sequence; recombinant DNA

Our laboratory is engaged in the following studies: 1) Elucidation of the structural and quantitative differences between rapid and slow N- acetyltransferases from humans and mice, the acetylator phenotype of which is determined both in vivo and in vitro, by: (a) assessment of the complete N-acetyltransferase (NAT) gene sequences; (b) comparison of the size, relative abundance, and stability of the corresponding NAT mRNAs, and (c) expression in heterologous systems, encompassing experiments on the kinetic and physicochemical properties of expressed variant NATs with those of the wild type; 2) Development of assays using biotinylated, allele-specific oligonucleotide probes for distinguishing rapid and slow human NAT genes.

本实验室从事以下研究：1)阐明 快氮和慢氮在结构和数量上的差异 来自人和小鼠的乙酰化转移酶，其乙酰化表型 它在体内和体外都是通过以下方式确定的：(A)评估 N-乙酰转移酶(NAT)基因全序列；(B)比较 相应NAT的大小、相对丰度和稳定性 和(C)在异源系统中的表达，包括 表达产物的动力学和理化性质的实验研究 变异NAT与野生型NAT；2)检测方法的发展 使用生物素化、等位基因特异的寡核苷酸探针 区分快速和缓慢的人类NAT基因。