MOLECULAR BIOLOGY OF BLOOD COAGULATION FACTORS

凝血因子的分子生物学

• 批准号: 6274667
• 负责人: KOTOKU KURACHI
• 金额: $ 2.15万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6274667
• 关键词: aging; coagulation factor IX; genetic regulation; genetic regulatory element; genetically modified animals; homeostasis; laboratory mouse; molecular biology; nucleic acid repetitive sequence

The long-term goal of this study is to establish the molecular mechanism underlying the regulation of the homeostasis system. The overall blood coagulation potential dramatically increases with aging. This may have a great clinical significance in the increased frequency of thrombotic and cardiovascular disorders with advancing age. As a first model gene for us to gain insights into the underlying mechanism, we have analyzed the relationship of the structure and age-associated regulation of the factor IX gene with transgenic mice. We have successfully identified a small 5' upstream region (about 400 bp) of the gene to be critical not only for its age-associated regulation, but also for its tight liver specific expression. This region is a part of the Line 1 repetitive sequence, thus identifying the novel biological functions for the repetitive sequence.

本研究的长期目标是建立分子机制 是体内平衡系统的基础。 整体血液 凝固潜力随着老化而显著增加。 这可能 血栓形成的频率增加具有很大的临床意义， 和心血管疾病的风险。 作为第一个模型基因 为了深入了解潜在的机制，我们分析了 的结构和年龄相关的调节的关系， 因子IX基因的转基因小鼠。 我们已经成功识别出 该基因的一个小的5'上游区域（约400 bp）是关键的， 不仅是因为它的年龄相关的调节，而且因为它的紧凑的肝脏， 具体表达。 此区域是第1行重复的一部分 序列，从而鉴定所述多肽的新生物学功能。 重复序列