Choosing Coral Winners

“NERC：EisethBeauchamp：NE/S 007512/1“我研究的中心目标是通过确定耐热性种群内变异的根本原因，为珊瑚礁的辅助进化干预提供信息。如果可以确定与耐热性增加相关的某些生理或遗传特征，这些可能在确定用于恢复和适应干预的最佳候选珊瑚中具有价值，这将是第一个检查自然热应力下选择性繁殖的F1珊瑚的共生藻科和原核微生物组的研究之一。Baum实验室最近发表了一项研究，该研究使用技术来识别珊瑚的共生藻科和微生物群落（ITS 2和16 s rRNA基因扩增子测序），这些珊瑚来自遥远的太平洋地区，与我自己在帕劳的研究地点有许多相似之处和一些关键的差异。通过这些方法，我将更好地了解共生藻科和原核微生物组在珊瑚全生物中的作用，加强研究，提高我的博士学位。这些F1珊瑚的分子分析提供了一个独特的机会，以了解表型耐热性及其相关的生理特性是如何通过几代人。这是建立辅助进化技术的可行性，涉及选择性育种的适应traits.I还建议访问不列颠哥伦比亚省的大学，以访问教授萨莉艾特肯，以及基林和Parfray实验室的。艾特肯教授是辅助进化应用的世界领导者，在那里我将获得有关表型特征和参与局部基因组适应的基因的知识。Keeling和Parfray实验室是促进微生物群落及其关系的额外培训的理想选择。在Baum实验室进行这个职位将为我提供一个宝贵的机会，为我的博士研究添加一个重要元素，并通过珊瑚分子生物学和生物信息学技术的培训来培养必不可少的技能。这也是一个独特的机会，可以在我的家乡研究所之外获得曝光，与世界领导人建立持久的网络和合作，同时建立作为研究人员的信心。

"NERC:ElizabethBeauchamp:NE/S007512/1"The central goal of my research is to inform assisted evolution interventions on coral reefs by determining the underlying causes of intra-population variation in heat tolerance. If certain physiological or genetic traits that associate with increased heat tolerance can be identified, these may be valuable in identifying the best candidate corals to be used for restoration and adaptation interventionsThis will be one of the first to examine the Symbiodiniaceae and the prokaryotic microbiome of selectively bred F1 corals under a natural heat stress. The Baum Lab have recently published research using techniques to identify Symbiodiniaceae and microbiome communities (ITS2 and 16s rRNA gene amplicon sequencing) of corals from a remote Pacific location that bears many similarities, and some crucial differences, to my own study location in Palau. Through these methods I will develop a better understanding of the role of Symbiodiniaceae and the prokaryotic microbiome within the coral holobiont, strengthening research, and enhancing my PhD. The molecular analysis of these F1 corals provides a unique opportunity to understand how phenotypic heat tolerance and its associated physiological traits are passed through generations. This is central to establishing the feasibility of assisted evolution techniques that involve selective breeding for adaptive traits.I also propose a visit to The University of British Columbia in order to visit Professor Sally Aitken, as well as the Keeling and Parfray Lab's. Professor Aitken is a world leader in the application of assisted evolution where I will gain knowledge about phenotypic traits and genes involved in local genomic adaption. The Keeling and Parfray Labs are an ideal choice to facilitate additional training in microbial communities and their relationships. Undertaking this placement at The Baum Lab will provide me with an invaluable opportunity to add an important element to my PhD research, and develop skills that are essential through training in coral molecular biology and bioinformatic techniques. This is also a unique chance to gain exposure outside of my home institute, form lasting networks and collaborations with world leaders whilst building confidence as a researcher.