Regulation of Calcium Impermeable AMPAR Subunit GluA2 in the Amygdala and Striatum of Cocaine-Seeking Rats by TARPs

TARPs 对寻求可卡因的大鼠杏仁核和纹状体中钙不可渗透的 AMPAR 亚基 GluA2 的调节

• 批准号: NE/V021214/1
• 负责人: David Belin
• 金额: $ 0.92万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FV021214%2F1
• 关键词: Regulation; Calcium; Impermeable; AMPAR; Subunit

The use of cocaine and heroin among young Europeans and Americans has been steadily increasing over the past decade (UN World Drug Report, 2018) and as such it has never been more important to understand the mechanisms whereby some individuals switch from recreational controlled drug use to compulsive drug seeking, the hallmark of drug addiction. This project aims to identify the mechanisms underlying a shift in network processing from recreational to habitual drug-seeking. We aim to identify proposed plasticity mechanisms in the amygdala that rely on modulations of gluatamate receptors. For this purpose, we will use brain tissue samples from habitually cocaine-seeking rats, as well as electrophysiology in slices and cultures of genetically modified mice. Combining these approaches will allow us to understand these mechanisms from a molecular, cellular, and behavioural perspective. Hereby we expect to find potential new drug targets for disengagement of the brain structure driving habitual drug-seeking behaviour.

过去十年来，欧洲和美国年轻人使用可卡因和海洛因的情况一直在稳步增加（联合国世界毒品报告，2018年），因此，了解一些人从娱乐性受控药物使用转向的机制从未如此重要。强迫性吸毒，这是吸毒成瘾的标志。该项目旨在确定网络处理从娱乐性转向习惯性寻求药物的潜在机制。我们的目的是确定建议的可塑性机制，在杏仁核依赖于谷氨酸受体的调制。为了这个目的，我们将使用习惯性吸食可卡因的大鼠的脑组织样本，以及转基因小鼠切片和培养物中的电生理学。结合这些方法将使我们能够从分子，细胞和行为的角度来理解这些机制。因此，我们希望找到潜在的新的药物靶点，以脱离大脑结构驱动习惯性药物寻求行为。