RELIABILITY/REPRODUCIBILITY OF MEASUREMENT OF CTL ACTIVITY IN HIV

这是一项评估可靠性和重复性的研究 盲人CTL活性和HIV特异性增殖的测定 15例HIV感染者和3例非HIV感染者对照研究 分别在0周和2周收集血液单个核细胞的证据 稍后和CTL检测HIV特异性活性以及HIV特异性 在波林格博士和莱德曼博士的研究中评估了增殖活性 实验室。所有18个拟议的科目都已完全登记并应计 为了这项研究。此外，艾滋病毒血浆病毒载量和细胞表面 在奎恩博士的实验室对所有患者进行了流式细胞仪分析 样本。这项研究的初步结果以摘要形式概述。 最近提交给在日内瓦举行的第121届世界艾滋病大会 1998年6月。总之，在这个盲目分析中，HIV特异性CTL 批量培养法检测多种HIV抗原的活性 冰冻PBMC抗原特异性刺激作用的统计学研究 与病毒载量和CD_4细胞计数显著相关。最好的方法 为了将HIV特异性CTL与临床分期相关联，利用了一个区域 在特定裂解曲线下进行分析。结论是，这是 该方法可用于临床试验中CTL活性的测定。

This is a study to assess the reliability and reproducibility of measurements of CTL activity and HIV-specific proliferation in a blinded study of 15 HIV infected and 3 HIV uninfected control individuals. Proof of blood mononuclear cells were collected at time 0 and two weeks later and CTL assays of HIV specific activity as well as HIV specific proliferative activity were assessed in Dr. Bollinger and Dr. Lederman's Lab. All 18 of the proposed subjects were fully enrolled and accrued for this study. In addition, HIV plasma viral load and cell surface flow cytometric analysis was performed in Dr. Quinn's lab on all samples. Preliminary results of this study are outlined in an abstract recently submitted to the 121 World AIDS Conference in Geneva scheduled for June, 1998. In summary, in this blinded analysis HIV-specific CTL activity directed against multiple HIV antigens measured by bulk culture antigen specific stimulation of frozen PBMC's demonstrated statistically significant correlation with viral load and CD4 count. The best method for correlating HIV specific CTL with clinical stage utilized an area under specific lysis curve analysis. The conclusion was that this method may be useful for measuring CTL activities in clinical trials.