NUCLEIC ACID VACCINATION AGAINST SIV (2)

猴免疫缺陷病毒(SIV)感染的意义 猕猴被认为是艾滋病最好的动物模型。尽管 尽管付出了巨大的努力，但还没有找到针对SIV的保护性疫苗。 目的本研究将检验核酸疫苗接种是否可以 产生对SIV感染的保护性免疫力。我们将使用 我们在第一部分中确定的最佳疫苗接种条件 本研究旨在为动物接种疫苗，并密切跟踪诱导免疫 回应。结果实验动物分为4组，每组4只。 每组的实验方案略有不同。动物 在实验开始和3个月时接种两次疫苗。 我们正在进行几种不同的测量 体液免疫和细胞免疫。第一个月后的八个月 注射后，动物将接受SIV口服挑战，并随后进行 又过了6个月。将对动物进行监测，以了解它们对 病毒感染和对疾病发展的抵抗力。未来 如果我们能够产生保护性免疫，下一步 实验问题将是宿主免疫的哪些成分能够 才能产生保护作用。第二个方向将是应用 结果生产出一种人类艾滋病毒疫苗。SIV；疫苗

Significance Simian Immunodeficiency Virus (SIV) infection of macaques is considered the best animal model for AIDS. Despite intense effort, no protective vaccine against SIV has been found. Objectives This study will test whether nucleic acid vaccination can produce protective immunity against SIV infection. We will use the optimal vaccination conditions that we determined in the first part of this study to vaccinate animals and closely follow the induced immune response. Results Four groups of four animals are used in this study. Each group has a slightly different experimental protocol. Animals are vaccinated twice, at the start of the experiment and at 3 months. We are performing several different measurements of the induced humoral and cellular immunity. Eight months after the first injection, animals will be challenged orally with SIV and followed for another 6 months. Animals will be monitored for both resistance to viral infection and resistance to the development of disease. Future Directions If we are able to produce protective immunity, the next experimental question will be what component of host immunity was able to produce the protection. A second direction will be applying the result ot produce a human HIV vaccine. KEYWORDS SIV, vaccine