NUCLEIC ACID VACCINATION AGAINST SIV (2)

SIV 核酸疫苗 (2)

• 批准号: 6277910
• 负责人: Murray Briggs Gardner
• 金额: $ 8.13万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277910
• 关键词: AIDS; Chordata; Mammalia; Primates; biomaterials; immunology; infection; inflammation; lymphatic system; sexually transmitted diseases; virus

Significance Simian Immunodeficiency Virus (SIV) infection of macaques is considered the best animal model for AIDS. Despite intense effort, no protective vaccine against SIV has been found. Objectives This study will test whether nucleic acid vaccination can produce protective immunity against SIV infection. We will use the optimal vaccination conditions that we determined in the first part of this study to vaccinate animals and closely follow the induced immune response. Results Four groups of four animals are used in this study. Each group has a slightly different experimental protocol. Animals are vaccinated twice, at the start of the experiment and at 3 months. We are performing several different measurements of the induced humoral and cellular immunity. Eight months after the first injection, animals will be challenged orally with SIV and followed for another 6 months. Animals will be monitored for both resistance to viral infection and resistance to the development of disease. Future Directions If we are able to produce protective immunity, the next experimental question will be what component of host immunity was able to produce the protection. A second direction will be applying the result ot produce a human HIV vaccine. KEYWORDS SIV, vaccine

猴免疫缺陷病毒（SIV）感染的意义 猕猴被认为是最好的艾滋病动物模型。 尽管 尽管人们付出了巨大的努力，但还没有发现预防SIV的疫苗。 目的本研究旨在验证核酸疫苗是否能 对SIV感染产生保护性免疫。 我们将使用 我们在第一部分确定的最佳疫苗接种条件 本研究对接种动物进行了诱导免疫并密切跟踪 反应 结果本实验共分4组，每组4只动物。 每个小组都有一个稍微不同的实验方案。 动物 在实验开始时和3个月时接种两次疫苗。 我们正在进行几种不同的测量， 体液免疫和细胞免疫。 在第一次世界大战后的八个月 注射后，将用SIV经口攻击动物，并随访 再过6个月。 将监测动物对以下两种药物的抗性： 病毒感染和抵抗疾病的发展。 未来 如果我们能够产生保护性免疫， 实验问题是宿主免疫的哪种成分能够 以产生保护。 第二个方向将是应用 这是一个生产人类艾滋病疫苗的结果。 关键词SIV，疫苗