NUCLEIC ACID VACCINATION AGAINST SIV

SIV 核酸疫苗接种

• 批准号: 6277911
• 负责人: Murray Briggs Gardner
• 金额: $ 8.13万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277911
• 关键词: AIDS; Chordata; Mammalia; Primates; biomaterials; immunology; infection; inflammation; lymphatic system; sexually transmitted diseases; virus

Significance Nucleic acid vaccination has several advantages over conventional protein immunization. This new method has been extensively tested in mice but there have been few tests of it in larger animals. Objectives The present study was carried out to optimize nucleic acid vaccination in primates. We have varied the dose of DNA and the injection volume using intramuscular immunization and tested the dose for intradermal vaccination. Results We measured both humoral and cellular immune responses after vaccination of each animal with three different antigens. We find that immune responses appear 2-3 months after vaccination which is much slower than that seen in rodents. However, surprisingly small amounts of DNA are needed to vaccinate a macaque. We find optimal DNA doses of 200 micrograms of DNA are needed for intramuscular injection and 50 micrograms for intradermal injection. Thus only a 5 fold increase in DNA is needed when comparing primates to rodents even though they differ in body weight by 600 times. The volume of injection is also important for intramuscular injection. The immunity induced in primates is comparable to the protective levels we see in rodents. Future Directions These experiments have established conditions needed for an efficient DNA vaccination in primates. We will use this knowledge in our next experiment to vaccinate animals against SIV. KEYWORDS SIV, vaccine, nucleic acid vaccine

核酸疫苗接种有几个优点， 常规蛋白免疫。 这种新方法已经 在老鼠身上进行了广泛的测试，但在 更大的动物。 目的本研究旨在 优化灵长类动物的核酸疫苗接种。 我们改变了 DNA的剂量和注射体积使用肌内免疫 并测试了皮内接种的剂量。 我们测量的结果 接种后的体液和细胞免疫应答 有三种不同抗原的动物 我们发现免疫反应 在接种疫苗后2-3个月出现，这比接种疫苗要慢得多。 在啮齿类动物中发现 然而，令人惊讶的是， 需要给猕猴接种疫苗 我们发现最佳DNA剂量为200 肌肉注射需要50微克的DNA， 皮内注射用微克。 因此，只有5倍的增长， 在将灵长类动物与啮齿类动物进行比较时， 体重相差六百倍 注射体积也是 对肌肉注射很重要。 诱导的免疫 灵长类动物的保护水平与我们在啮齿类动物中看到的保护水平相当。 这些实验已经建立了所需的条件 在灵长类动物中进行有效的DNA疫苗接种。 我们将使用这个 在我们的下一个实验中为动物接种SIV疫苗。 关键词SIV，疫苗，核酸疫苗