IMMUNOPHENOTYING OF LYMPHOCYTES FROM NEWBORN & INFANT RHESUS MACAQUES

新生儿淋巴细胞的免疫表型

• 批准号: 6277918
• 负责人: PAUL A LUCIW
• 金额: $ 8.13万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277918
• 关键词: AIDS; Mammalia; Primates; immunology; infection; inflammation; lymphatic system; virus

Significance In this project on newborn (neonate) rhesus macaques followed for the first year of life, data collected on numbers and ratios of lymphoid cell subsets in peripheral blood provided baseline information on immune cell subsets in normal healthy animals. Comparisons of similar data on immune cell subsets can now be made in newborn and infant macaques infected with various viruses including simian immunodeficiency virus (SIV), simian-human immunodeficiency virus chimras (SHIV), rhesus cytomegalovirus (RhCMV), and measles virus. Objectives The objective was to develop baseline parameters for several lymphoid cell subsets in peripheral blood of newborn rhesus macaques by flow cytometry. Seven antibody markers to cell surface differentiation and activation antigens were used in this longitudinal study as follows CD2 (Coulter) for T cells and NK cells; CD20 (Becton-Dickinson) for B cells; CD4 (Ortho) for helper/inducer T cells; CD8 (Becton-Dickinson) for cytotoxic/suppressor T-cells; CD14 (Becton-Dickinson) for monocytes, granullocytes, macrophages, and dendritic cells; CD25 (Becton-Dickinson) for interleukin-2 receptor; HLA-DR (Becton-Dickinson) for B cells, monocytes, macrophages, activated T cells, and NK cells. Results Peripheral blood was collected from six healthy uninfected newborn rhesus macaques at weekly and monthly intervals for the first year of life. Flow cytometry analysis was performed with the seven marker antibodies listed above. These data have been made available to other investigators analyzing the effects of various viruses (SIV, SHIV, RhCMV, measles virus) on immune cell subsets in pathogenesis and vaccine studies. Future Directions New markers are becoming available for characterizing peripheral blood immune cells in rhesus macaques. These markers will allow specific identification of dendritic cells, T naive and T memory cells, monocytes, cell cycle and novel activation markers, etc. KEYWORDS SIV, immune system

本项目对新生(初生)恒河猴的意义 在生命的第一年进行跟踪，收集关于数字和 提供基线的外周血中淋巴细胞亚群比率 关于正常健康动物免疫细胞亚群的信息。 关于免疫细胞亚群的相似数据的比较现在可以在 新生和婴儿猕猴感染了各种病毒，包括 猴免疫缺陷病毒(SIV)，猿人免疫缺陷 病毒嵌合体(SHIV)、恒河猴巨细胞病毒(RhCMV)和麻疹 病毒。目标是开发基线参数 新生儿外周血中几种淋巴细胞亚群的检测 用流式细胞仪检测恒河猴。七种细胞抗体标记物 表面分化和激活抗原在这个过程中使用 纵向研究：T细胞和NK细胞CD2(Coulter)； CD20(Becton-Dickinson)用于B细胞；CD4(Ortho)用于辅助/诱导T细胞 细胞；细胞毒/抑制性T细胞的CD8(Becton-Dickinson)；CD14 (Becton-Dickinson)，用于单核细胞、粒细胞、巨噬细胞和 树突状细胞；CD25(Becton-Dickinson)； B细胞、单核细胞、巨噬细胞、 活化的T细胞和NK细胞。结果外周血 采集自6只健康的未受感染的新生恒河猴 生命第一年的每周和每月间隔。流动 用这7种标志物抗体进行细胞分析 上面列出了。这些数据已提供给其他人 研究人员分析了各种病毒(SIV，SIV，SHIV， 在发病机制中对免疫细胞亚群的影响 疫苗研究。未来方向新的标记器正在出现 用于鉴定猕猴的外周血液免疫细胞。 这些标记将允许对树突状细胞、T细胞进行特异性鉴定 幼稚和T记忆细胞、单核细胞、细胞周期和新的激活 标志物等。SIV，免疫系统