BACTERIAL GENOME RESTRICTION DIGEST ANALYSIS: FLOW CYTOMETRY DNA FRAGMENT SIZING

细菌基因组限制性酶切分析：流式细胞术 DNA 片段大小测定

• 批准号: 6280899
• 负责人: JAMES H JETT
• 金额: $ 1.31万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280899
• 关键词: bacteria; biomedical equipment development; biomedical resource; technology /technique

DNA fragment size analysis by flow cytometry has been applied to the analysis of restriction enzyme digenst of the S. aureus genome. Of the 14 fragments generated by Sma I digestion, 13 have been observed up to 360 kilobase pairs in length. This represents an extension of a factor of two of the range of fragment sizes that can be analyzed by flow cytometry. More details are contained in the Research Highlight section of this report.

流式细胞术已应用于DNA片段大小分析