SYNCHROTRON CRYSTALLOGRAPHY OF ENZYMES AT BEAMLINE X 9B: HIV, DRUG TARGET

光束线 X 9B 上酶的同步晶体学：HIV、药物靶标

• 批准号: 6120424
• 负责人: ZBIGNIEW DAUTER
• 金额: $ 0.34万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120424
• 关键词: AIDS; biological products; biomedical resource; enzymes; immunology; infection; inflammation; lymphatic system; proteins; spectrometry; virus

Rhoa complex. The crystals diffracted weakly to about 4.5A resolution. 140 frames were collected with oscillation of 0.5deg per frame and 30 seconds of exposure time. Due to the anisomorphism of the diffraction pattern the data are complete only to 6A resolution. The overall completeness of the data is 86.2%. Rsym for the data is 12% with the overall redundancy of 6. Overall I/sigma is 11.0 (1.9 in the last resolution shell). The data is currently used in molecular replacement attempts in order to solve the structure. PAF Acetylhydrolase mutant R22K. The data were collected to 1.9A resolution. The overall completeness of the data is 90%. With the redundancy of 5.4 the Rsym is 7% in resolution range from 20A to 1.9A. The structure is in the final stages of crystallographic refinement.

罗亚情结。晶体的衍射率约为4.5A。 决议。采集了140帧，摆动速度为0.5度/秒 帧和30秒的曝光时间。由于同构的原因 数据的衍射图仅在6A分辨率下才完整。 数据的总体完备率为86.2%。数据的Rsym为 12%，总体冗余度为6。总体I/Sigma为11.0(1.9英寸 最后一个分辨率外壳)。该数据目前用于分子 为了解决结构问题，人们试图进行替换。PAF 乙酰水解酶突变体R22K。数据收集到1.9A 决议。数据的总体完备率为90%。与 5.4的冗余度Rsym在20A到1.9A的分辨率范围内为7%。 该结构正处于晶体提纯的最后阶段。