CCD DETECTOR OPERATION FOR PROTEIN CRYSTALLOGRAPHY ON X9B

X9B 上蛋白质晶体学的 CCD 探测器操作

• 批准号: 6205771
• 负责人: ZBIGNIEW DAUTER
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205771
• 关键词: biological products; biomedical resource; proteins; spectrometry

Time-resolved structure determinations are at the forefront of attempts to understand the mechanisms of protein reactions. We have demonstrated a system for collecting high quality x-ray absorption spectroscopy data on microsecond timescales. The method can be used for both full spectra accumulation and kinetic spectro-photometry. This system has been used to examine the formation of triplet states in zinc and platinum substituted proto- and octaethyl prophyrins, where the excited state structure is of intense theoretical interest. Preliminary data show little structural change in the excited state, implying only minor movement of the metal out of the porphyrin plane upon photo-excitation.

时间分辨结构测定处于最前沿 试图了解蛋白质反应的机制。 我们有 展示了一种收集高质量X射线吸收的系统 微秒时间尺度上的光谱数据。 该方法可用于 用于全光谱积累和动力学分光光度法。 该系统已被用于检查的形成 三重 国 在锌和铂取代的原代和八乙基藜芦碱中， 其中激发态结构具有强烈的理论兴趣。 初步数据显示激发态的结构变化很小， 这意味着金属仅轻微地移动出卟啉平面 在光激发下。