STRUCTURE & FUNCTION ANALYSIS OF TOLEROGENIC PEPTIDES

结构

• 批准号: 6281187
• 负责人: JOHN H GRIFFIN
• 金额: $ 0.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281187
• 关键词: animal tissue; biological products; biomaterials; biomedical resource; drug /agent; human tissue; immunology; inflammation; lymphatic system; model design /development; spectrometry; structural biology; technology /technique; transplantation

We have undertaken a project in collaboration with colleagues in Stanford University School of Medicine to study peptides derived from MHC molecules which exhibit interesting immunomodulatory properties. The long-term goals of this effort are to understand in detail the basis for induction of T cell tolerization by these peptides, and also to develop potential candidates for use in human clinical immunsuppressive therapy. Our role is to carry out a structure/function analysis of MHC-derived peptides in order to determine the structural elements that are necessary and sufficient for immunosuppressive activity. To date, we have found, through a "serine scane" amino acid residues of a base sequence dodecapeptide which are critical for activity. One of these derivatives, and two other analogs have activity superior to that of the parent sequence. We plan to continue these studies, and to use tagged peptides as affinity ligands for receptor purification and biophysical characterization. FAB-MS is n invaluable tool for confirming the structure of the peptides and peptide derivatives that we synthesize in this

我们与英国的同事合作进行了一个项目