SINGLE MOLECULES ON CELL MEMBRANES

荧光相关光谱法非常适合于 单个荧光分子的检测和分析，例如 通过单个罗丹明B的荧光猝发的时间轨迹 分子扩散通过激发体积。 FCS使用 双光子激发提供了数学上 焦点体积的明确定义的三维几何形状。 期间 单个分子通过激发扩散的每个事件 体积，荧光的爆发可以分析相对于快速 具有高统计精度的动力学过程。 我们调查 通过FCS和爆发分析在细胞膜中的单个OD-TMR分子 多通道标量。

Fluorescence correlation spectroscopy is ideally suited for detection and analysis of single fluorescent molecules, as exemplified by time traces of fluorescence bursts of individual rhodamine B molecules diffusing through the excitation volume. FCS using two-photon excitation provides the advantage of a mathematically well-defined three-dimensional geometry of the focal volume. During each event of a single molecule diffusing through the excitation volume, the burst of fluorescence can be analyzed in respect to fast kinetic processes with high statistical accuracy. We investigated single OD-TMR molecules in cell membranes by FCS and burst analysis with a multichannel scalar.