APPLICATION OF LIFETIME IMAGING TO DRUG LOCALIZATION & ACTIVATION: RAT TISSUE

肿瘤导向的药物输送系统正在老鼠身上进行测试。 荧光抗肿瘤药物的使用允许对 扫描技术用于药物组织靶向的有效性研究 技巧。组织切片的强度扫描指示靶向 这些药物被转移到肿瘤组织，但药物也在 在小鼠组织和小鼠器官中。药物的激活诱导了一种 荧光寿命的变化表明寿命扫描是一种 区分非活动性和非活动性局部化的潜在方法 活性药物。与ISS(伊利诺伊州香槟)联合开发原型 扫描装置被制作成与标准ISS阶段一起工作， 调制荧光计。然后扫描保存下来的大鼠组织切片 以获取强度和荧光寿命信息。相位对比度 清楚地显示了组织的差异，并提供了初步的 生命周期成像将有助于区分毒品的证据 从药物积累中激活。

Tumor-directed drug delivery systems are being tested in mice. The use of fluorescent anti-tumor drugs has permitted evaluation of the effectiveness drug tissue targeting through the use of scanning techniques. Intensity scanning of tissue slices indicated targeting the drugs to tumor tissue but drugs were also found throughout the mouse tissue and in mouse organs. Activation of the drug induces a fluorescence lifetime change which suggested lifetime scanning as a potential method to discriminate between localization of inactive and active drug. In conjunction with ISS (Champaign, IL) a prototype scanning device was made to work with a standard ISS phase and modulation fluorometer. Preserved rat tissue slices were then scanned for intensity and fluorescence lifetime information. Phase contrast clearly indicated differences in tissues and provided preliminary evidence that lifetime imaging would be useful in discriminating drug activation from drug accumulation.