UPAR-UPA BINDING STOICHIOMETRY IN LIVE CELLS

活细胞中的 UPAR-UPA 结合化学计量

• 批准号: 8170957
• 负责人: VALERIA CAIOLFA
• 金额: $ 1.61万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170957
• 关键词: Binding; Cell Adhesion; Cell Line; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Kinetics; Life; Ligands; Physiological; Research; Research Personnel; Resources; Series; Source; Testing; United States National Institutes of Health; novel; overexpression; receptor; receptor binding; receptor internalization; stoichiometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This projects aims at elucidating the binding stoichiometry of uPAR, its main physiological ligand, uPA, and modulators of its activity in cellular adhesion mechanisms. The stoichiometry will be studied by the novel N&B approach in live cells using GFP-tagged receptor and ligands. A series of cell lines expressing GFP-uPAR or mCherry-uPAR will be tested for investigating the effect of overexpression of the receptor and the binding kinetics and internalization of the receptor-ligand complexes.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目旨在阐明uPAR、其主要生理配体uPA及其在细胞粘附机制中的活性调节剂的结合化学计量。 化学计量学将通过新的N&B方法在活细胞中使用GFP标记的受体和配体来研究。 将检测一系列表达GFP-uPAR或mCherry-uPAR的细胞系，以研究受体过表达的影响以及受体-配体复合物的结合动力学和内化。