UPAR-UPA BINDING STOICHIOMETRY IN LIVE CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本项目旨在阐明uPAR及其主要生理配体uPA的结合化学计量学，以及其在细胞黏附机制中的活性调节因子。 化学计量学将在活细胞中使用GFP标记的受体和配体通过新的N&B方法进行研究。 将测试一系列表达GFP-uPAR或mCherry-uPAR的细胞系，以研究受体过表达的影响以及受体-配体复合体的结合动力学和内化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This projects aims at elucidating the binding stoichiometry of uPAR, its main physiological ligand, uPA, and modulators of its activity in cellular adhesion mechanisms. The stoichiometry will be studied by the novel N&B approach in live cells using GFP-tagged receptor and ligands. A series of cell lines expressing GFP-uPAR or mCherry-uPAR will be tested for investigating the effect of overexpression of the receptor and the binding kinetics and internalization of the receptor-ligand complexes.