POLYCRYSTALLINE ESEEM OF CU(II) INSULIN

铜(II)胰岛素的多晶ESEEM

• 批准号: 6281730
• 负责人: MICHAEL COLANERI
• 金额: $ 3.12万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-05 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281730
• 关键词: bioengineering /biomedical engineering; biological products; biomaterials; biomedical equipment development; biomedical resource; technology /technique

An ESEEM investigation of Cu(II)-Insulin in polycrystalline samples has revealed that the quadrupole parameters for the remote nitrogen of the two equatorial HIS imidazoles bound to copper are similar to those found in type I copper proteins. When Cd(II) ions are allowed to soak into these crystalline samples a dramatic switch is observed in these parameters, which change to values more similar to those found in type II copper sites. FT-ESEEM simulations based on atomic positions derived from Insulin crystal structures show that this switch is due to a change in the hydrogen bonding environment of the remote nitrogen.

Cu（II）-胰岛素多晶的ESEEM研究 样品显示，四极参数的远程 与铜结合的两个赤道HIS咪唑的氮是 类似于I型铜蛋白中发现的那些。 当Cd（II）离子 被允许渗入这些晶体样本中， 在这些参数中观察到，这些参数的变化值更相似 与II型铜矿中发现的那些不同。 FT-ESEEM模拟基于 胰岛素晶体结构的原子位置表明， 这种转变是由于氢键环境的改变， 遥远的氮气