ELECTRON SPIN ECHO STUDIES OF RUSTICYANIN

记录了蓝色分子的电子自旋回波调制谱 铜蛋白rusticyanin和一个缺乏这两者之一的突变体 组氨酸配体到铜(H85A)。W.T.和H85A表格 对蛋白质进行了深入的研究，以充分表征ESEEM 咪唑氮基在铜配体中的光谱性质 菲尔德。三个脉冲中定时变量(tau选择)的使用 已经建立了一种识别峰的实验方法。一个 识别峰和量化超精细的图解方法 实验数据中的参数也得到了验证。这个 ESEEM谱表明，两个自旋流形的m=1跃迁可能 在三脉冲实验中，通过明智地选择tau来观察。 这大大提高了模拟过程的准确性，并 消除通过不正确的关联错误识别峰的模糊性。 峰值的图形表示使人们能够预测 “精确取消”的条件，从而消除 对超精细参数进行量化。此外，由于没有 第二个组氨酸配体在剩余的组氨酸上增加了更多的自旋密度 组氨酸配基为铜。在此基础上发表了论文

Electron spin echo modulation spectra were recorded for the blue copper protein rusticyanin and a mutant lacking one of the two histidine ligands to copper (H85A). The w.t. and H85A forms of the protein were examined in depth to fully characterize the ESEEM spectroscopic properties of imidazole nitrogens in the copper ligand field. The use of timing variables (tau selection) in the three pulse experiment as a means to identify peaks has been established. A graphical method of identifying peaks and quantifying superhyperfine parameters from experimental data has also been demonstrated. The ESEEM spectra suggest that m=1 transitions of both spin manifolds may be observed by a judicious choice of tau in a three pulse experiment. This greatly improves the accuracy of the simulation process and removes ambiguity in misidentifying peaks by an improper association. A graphical representation of the peaks allows one to predict the condition of "exact cancellation" and thereby remove ambiguities in quantifying the superhyperfine parameters. Further, the absence of the second histidine ligand puts more spin density onto the remaining histidine ligand to copper. Paper has been published based on this