EFFECTS OF BLEOMYCIN ON AIDS ASSOCIATED CANDIDA ALBICANS: CELL WALL STRUCT

博莱霉素对艾滋病相关白色念珠菌的影响：细胞壁结构

• 批准号: 6121538
• 负责人: CAROL W MOORE
• 金额: $ 9.98万
• 依托单位: CITY COLLEGE OF NEW YORK
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121538
• 关键词: AIDS; biotechnology; immunology; infection; inflammation; lymphatic system; minority institution research support; plants; structural biology; virus

Fungal infections are a serious problem in the clinical management of AIDS patients. Without treatment targeted to the pathogenic fungi, they are often fatal to AIDS patients. The long term objectives of this project are to determine and understand the basic mechanism of action of the cell wall effects of bleomycin on the AIDS opportunistic fungal pathogen, Candida albicans. The specific aims are to determine some of the cellular, molecular and biochemical bases for cell wall effects of bleomycin in C. albicans, and determine which components of the C. albicans cell wall react wit bleomycin. Using several experimental approaches, we will determine the range of susceptibilities of C. albicans isolates to killing, cell wall damage, and chromosomal breaks caused by bleomycin treatments, and determine specific changes in the cell wall associated with bleomycin treatments using transmission electron microscopy, biochemical studies of cell wall components, and pulsed-field gel electrophoresis for assays of dna breaks and electrophoretic karyotyping. The research is designed to determine the basic mechanism of the antifungal action f bleomycin on C. albicans cell walls. This basic information should lead to an understanding of the basic biology of C. albicans isolates and therapeutic usefulness of low doses of bleomycin or an analog as an antifungal for acute treatments. Low doses of the drug or an analog may be able to be used as a single agent or in one or more drug combinations, particularly when C. albicans develops resistance to another antifungal drug. The information from this research should also lead to the identification of potential target structures for additional antifungal drugs and assist in antifungal drug design and therapy to target critical structures.

真菌感染是临床管理中的一个严重问题