TH1 CYTOKINE DEFICIENCIES IN CATS INFECTED WITH FIV

感染 FIV 的猫的 TH1 细胞因子缺乏

• 批准号: 2703167
• 负责人: JULIE K LEVY
• 金额: $ 7.57万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2703167
• 关键词: HIV infections; Toxoplasma gondii; antibody; cats; cellular immunity; cytokine receptors; disease /disorder model; feline immunodeficiency virus; gene expression; immune tolerance /unresponsiveness; immunodeficiency; interferon gamma; interleukin 12; lymph nodes; messenger RNA; nucleic acid sequence; polymerase chain reaction; tissue /cell culture; toxoplasmosis; tumor necrosis factor alpha; virus infection mechanism

DESCRIPTION (Adapted from the applicant's abstract): Animal models, such as FIV, are critical to understanding the mechanism of HIV-induced immunodeficiency. The model can be used to dissect the molecular basis of lentivirus-associated immunodeficiency. Preliminary work by the candidate has shown that FIV, like HIV, induces an early and profound deficiency in IL-12 expression, which is associated with susceptibility to T. gondii. What is not yet known is whether this deficiency results from a direct effect of FIV, or whether the virus suppresses critical IL-12 priming cytokines such as TNF(alpha) or IFN(gamma). This application proposes to identify the sequence of protective Type one cytokine suppression. The candidate will also test the hypothesis that exogenous IL-12 administration will reconstitute the cell-mediated immune response, and protect FIV-infected cats from T. gondii challenge. As a veterinary internist and immunologist, Dr. Levy is committed to a career as a clinical investigator in an academic institution. The Co-sponsors of this candidate are authorities in feline retroviral research. The laboratories in which the candidate is engaged are capable of providing the space, equipment, animal facilities, and support staff required for successful completion of the application.

描述（改编自申请人的摘要）：动物模型，如 FIV，对于理解HIV诱导的 免疫缺陷。 该模型可用于解剖的分子基础， 慢病毒相关免疫缺陷 候选人的初步工作 已经表明，FIV，像艾滋病毒，诱导早期和深刻的缺陷， IL-12表达与T.刚地。 目前尚不清楚的是，这种缺陷是否是由直接的 FIV的影响，或病毒是否抑制关键的IL-12引发 细胞因子如TNF（α）或IFN（γ）。 本申请建议 确定保护性1型细胞因子抑制的序列。 的 候选人还将检验外源性IL-12给药 将重建细胞介导的免疫反应， FIV感染猫T.弓形虫感染。 作为一名兽医内科医生， 作为一名免疫学家，Levy博士致力于临床研究者的职业生涯 在一个学术机构。 该候选人的共同发起人是 猫逆转录病毒研究的权威。 实验室中， 应聘者有能力提供空间、设备、动物 设施和支助人员， 应用程序.