TH1 CYTOKINE DEFICIENCIES IN CATS INFECTED WITH FIV

感染 FIV 的猫的 TH1 细胞因子缺乏

• 批准号: 6188672
• 负责人: JULIE K LEVY
• 金额: $ 9.08万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188672
• 关键词: HIV infections; Toxoplasma gondii; antibody; cats; cellular immunity; cytokine receptors; disease /disorder model; feline immunodeficiency virus; gene expression; immune tolerance /unresponsiveness; immunodeficiency; interferon gamma; interleukin 12; lymph nodes; messenger RNA; nucleic acid sequence; polymerase chain reaction; tissue /cell culture; toxoplasmosis; tumor necrosis factor alpha; virus infection mechanism

DESCRIPTION (Adapted from the applicant's abstract): Animal models, such as FIV, are critical to understanding the mechanism of HIV-induced immunodeficiency. The model can be used to dissect the molecular basis of lentivirus-associated immunodeficiency. Preliminary work by the candidate has shown that FIV, like HIV, induces an early and profound deficiency in IL-12 expression, which is associated with susceptibility to T. gondii. What is not yet known is whether this deficiency results from a direct effect of FIV, or whether the virus suppresses critical IL-12 priming cytokines such as TNF(alpha) or IFN(gamma). This application proposes to identify the sequence of protective Type one cytokine suppression. The candidate will also test the hypothesis that exogenous IL-12 administration will reconstitute the cell-mediated immune response, and protect FIV-infected cats from T. gondii challenge. As a veterinary internist and immunologist, Dr. Levy is committed to a career as a clinical investigator in an academic institution. The Co-sponsors of this candidate are authorities in feline retroviral research. The laboratories in which the candidate is engaged are capable of providing the space, equipment, animal facilities, and support staff required for successful completion of the application.

描述(改编自申请人的摘要)：动物模型，如 FIV是理解HIV诱导的机制的关键 免疫缺陷。该模型可以用来剖析分子基础。 慢病毒相关性免疫缺陷。候选人的初步工作 已经表明，FIV和HIV一样，会导致早期和严重的 IL-12的表达与弓形虫的易感性有关。 目前尚不清楚的是，这种缺陷是否直接导致了 FIV的作用，或病毒是否抑制关键的IL-12启动 细胞因子，如肿瘤坏死因子(α)或干扰素(γ)。此应用程序建议 鉴定保护性1型细胞因子抑制序列。这个 候选人还将测试外源性IL-12注射的假设 将重建细胞介导的免疫反应，并保护 来自弓形虫的FIV感染猫的挑战。作为一名兽医内科医生 免疫学家，利维博士致力于临床研究人员的职业生涯 在一所学术机构工作。这位候选人的共同赞助人是 猫类逆转录病毒研究的权威人士。所在的实验室 应聘者有能力提供场地、设备、动物 成功完成项目所需的设施和支持人员 申请。