MULTIFREQUENCY AND HIGH FREQUENCY EPR OF SPIN-PROBES

描述(改编自申请人的摘要)：的总体目标 本修订提案将伊利诺伊州EPR研究中心(IERC)续期为 NIH研究资源中心将帮助有价值的研究，特别是 生物医学研究，通过开发、建立和提供特殊的 与电子顺磁共振有关的资源、方法和服务 光谱学(EPR)，特别强调多频和 高频EPR。IERC的具体目标是提供 科学，特别是生物医学，在以下方面提供援助 EPR在他们的研究问题中的应用。这包括(1)协作 以及需要进入EPR设施的服务(常规和特殊 最先进的)和专业知识，(2)技术研究和开发 作为仪器、方法和应用的演示，(3)培训 实用技术，以及(4)在全国范围内广泛传播 各种可能对EPR-Active生物医学有用的信息 科学界。IERC的特殊技术包括： 多频EPR和来自极低(无线电)的双磁共振 频率)DNP到高(毫米波接近远红外)光谱仪频率- 有许多与健康相关的应用，例如，改进制药 用于诊断放射学和确定有关结构和动力学的细节 在蛋白质和DNA中。重中之重的技术研发 在即将到来的赠款期间，重点将是高频和 蛋白质和DNA结构和动力学的多频率电子顺磁共振研究 自旋标记，特别是定点突变和定点自旋 贴标签。第二优先的技术研发重点将是 电子顺磁共振的发展及其在金属离子结构和动力学研究中的应用 具有生物相关性的自由基。八项技术研究与开发 项目详细说明了特定的应用程序，集中于这些优先事项和 将添加一些额外的工具来支持它们。这些部分 为了回应以前审查员的关切，对其进行了修订和扩充。 在续展期间，广泛传播、培训和服务 将继续开展活动。这些活动包括就以下问题举办一些国家讲习班 高频EPR方法，发布实质性时事通讯服务 对EPR感兴趣的生物医学和其他研究团体的成员， 通过维护IERC支持一个庞大的科学家网络 数据库和互联网信息网站，并为信息中心提供服务 交换信息。

DESCRIPTION (Adapted from the applicant's abstract): The broad objective of this revised proposal for renewal of the Illinois EPR Research Center (IERC) as an NIH Research Resource Center is to aid meritorious research, especially biomedical research, by developing, establishing, and providing special resources, methodology, and services related to electron paramagnetic resonance spectroscopy (EPR), with particular emphasis on multifrequency and high-frequency EPR. The specific aims of the IERC are to provide the scientific, especially biomedical, research community with assistance in applications of EPR to their research problems. This includes (1) collaboration and service requiring access to EPR facilities (both routine and special state-of-the art) and expertise, (2) technical research and development as well as demonstration of instrumentation, methods, and applications, (3) training in useful techniques, and (4) extensive national worldwide dissemination of a wide variety of information that might be useful to the EPR-active biomedical scientific community. Special techniques of the IERC include these: multifrequency EPR and double magnetic resonance from very low (radio frequency) DNP to high (mm-wave approaching far-IR) spectrometer frequencies- with many health-related applications, for example, improving pharmaceuticals for diagnostic radiology and determining details about structure and dynamics in proteins and DNA. The first-priority Technological Research and Development focus in the coming grant period will be the study by high frequency and multiple-frequency EPR of structure and dynamics in proteins and DNA through spin labeling, especially site-directed mutagenesis and site-directed spin labeling. The second-priority Technological Research and Development focus will be EPR development and applications to structure and dynamics of metal ions and radicals of biological relevance. Eight Technical Research and Development projects detail specific applications concentrating on these priorities and some additional instrumentation will be added to support them. These sections are revised and expanded in response to previous reviewers' concerns. During the renewal period, extensive dissemination, training, and service activities will be pursued. These include conducting some national workshops on high-frequency EPR methods, publishing a substantial Newsletter to serve members of the biomedical and other research community interested in EPR, supporting a large network of scientists through maintenance of an IERC database and Internet information site, and serving a clearing house for the exchange of information.