PULSED X BAND EPR SPECTROMETER FOR BIOMEDICAL RESEARCH

用于生物医学研究的脉冲 X 波段 EPR 光谱仪

• 批准号: 6291978
• 负责人: R LINN BELFORD
• 金额: $ 48.88万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291978
• 关键词: biomedical equipment purchase; electron spin resonance spectroscopy

X-band pulsed capability will be added to the extensive assortment of electron magnetic resonance instrumentation at the University of Illinois at Urbana/Champaign. This will provide the large and strong biomedical research community on this campus, including the nine named major- user groups, with a much needed state-of-the art tool for structural biology. The instrument will be sited in Illinois EPR Research Center (IERC) space within the School of Chemical Sciences and will be operated and managed by the IERC. The instrument chosen for this upgrade is the Bruker X-band ELEXYS FT-EPR Spectrometer System, which will incorporate the existing CW Bruker 300-series EPR spectrometer. The ELEXYS E580 FT-EPR spectrometer is a new- generation state-of-the-art instrument that is capable of performing almost all types of modern CW and pulsed EPR experiments at X-band. Dr. Sergei Dikanov, who is one of the world's leading experts in advanced pulsed electron magnetic resonance and its applications to biomedical problems involving metalloproteins and radicals and who has extensive direct experience with the E580 spectrometer system, will be in direct charge of operation and maintenance and will assist all users in effective design and interpretation of their research projects using this instrumentation. The nine major user projects focus on the following topics: contrast agents for diagnostic MRI (projects #1, Prof. R.B. Clarkson, and #2, Prof. P. Petillo); protein folding intermediates (project #3, Profs. A.I. Smirnov and R.L. Belford); electron transfer in bc1 complex (Prof. A.R. Crofts and Dr. S. Dikanov); ubiquinol oxidases (Prof R.B. Gennis), metalloenzymes protein design and engineering (Prof Y. Lu); mechanisms in vitamin B12-dependent enzymes, cytochrome c oxidase, and prostaglandin synthase (Prof. W. van der Donk); protein microspheres and heme protein analogs (Prof. K.S. Suslick); and hyperthermostable archaeal Rieske-type proteins (Dr. S. Dikanov). Many other significant users are expected from both inside and outside this institution. As examples, four minor user projects are described: insulin memesis by vanadium (Prof. C. Orvig, U. British Columbia); structure-function relationships in cytochrome c P-450 systems (Prof S. Sligar, U. Illinois); structure of NO-heme complex in irreducible and neuronal NO synthases (Dr. D. Stuehr, Cleveland Clinic); coordination geometry of dinitrosyl iron complexes in monomeric, reduced dimeric, and protein-bound forms (A. Vanin, Russ. Acad. Sci.).

X 波段脉冲功能将被添加到伊利诺伊大学厄巴纳/香槟分校的各种电子磁共振仪器中。这将为该校园内庞大而强大的生物医学研究社区（包括九个指定的主要用户组）提供结构生物学急需的最先进的工具。该仪器将安装在伊利诺伊州 EPR 研究中心 (IERC) 化学科学学院内，并由 IERC 运营和管理。此次升级选择的仪器是布鲁克 X 波段 ELEXYS FT-EPR 光谱仪系统，该系统将整合现有的 CW Bruker 300 系列 EPR 光谱仪。 ELEXYS E580 FT-EPR 光谱仪是新一代最先进的仪器，能够在 X 波段执行几乎所有类型的现代 CW 和脉冲 EPR 实验。 Sergei Dikanov 博士是先进脉冲电子磁共振及其在涉及金属蛋白和自由基的生物医学问题中的应用领域的世界领先专家之一，对 E580 光谱仪系统拥有丰富的直接经验，他将直接负责操作和维护，并协助所有用户使用该仪器有效设计和解释其研究项目。九个主要用户项目重点关注以下主题：用于诊断 MRI 的造影剂（项目#1，R.B. Clarkson 教授，项目 #2，P. Petillo 教授）；蛋白质折叠中间体（项目#3，A.I. Smirnov 和 R.L. Belford 教授）； bc1 复合物中的电子转移（A.R. Crofts 教授和 S. Dikanov 博士）；泛醇氧化酶（R.B. Gennis 教授）、金属酶蛋白质设计与工程（Y. Lu 教授）；维生素 B12 依赖性酶、细胞色素 C 氧化酶和前列腺素合酶的机制（W. ​​van der Donk 教授）；蛋白质微球和血红素蛋白类似物（K.S. Suslick 教授）；和超热稳定古菌 Rieske 型蛋白（S. Dikanov 博士）。预计该机构内部和外部还有许多其他重要用户。作为示例，描述了四个小型用户项目：钒的胰岛素模因（C. Orvig 教授，不列颠哥伦比亚大学）；细胞色素 c P-450 系统中的结构-功能关系（S. Sligar 教授，美国伊利诺伊州）；不可还原和神经元 NO 合酶中 NO-血红素复合物的结构（D. Stuehr 博士，克利夫兰诊所）；单体、还原二聚体和蛋白质结合形式的二亚硝基铁配合物的配位几何结构（A. Vanin, Russ. Acad. Sci.）。

项目成果

Characterization of mutants that change the hydrogen bonding of the semiquinone radical at the QH site of the cytochrome bo3 from Escherichia coli.

改变大肠杆菌细胞色素 bo3 QH 位点半醌基氢键的突变体的表征。

• DOI: 10.1074/jbc.m611595200
• 发表时间: 2007
• 期刊: The Journal of biological chemistry
• 作者: Yap,LaiLai;Samoilova,RimmaI;Gennis,RobertB;Dikanov,SergeiA
• 通讯作者: Dikanov,SergeiA

Characterization of the exchangeable protons in the immediate vicinity of the semiquinone radical at the QH site of the cytochrome bo3 from Escherichia coli.

大肠杆菌细胞色素 bo3 QH 位点半醌自由基附近可交换质子的表征。

• DOI: 10.1074/jbc.m602544200
• 发表时间: 2006
• 期刊: The Journal of biological chemistry
• 作者: Yap,LaiLai;Samoilova,RimmaI;Gennis,RobertB;Dikanov,SergeiA
• 通讯作者: Dikanov,SergeiA

Hydrogen bonds between nitrogen donors and the semiquinone in the Q(B) site of bacterial reaction centers.

氮供体和细菌反应中心 Q(B) 位点的半醌之间的氢键。

• DOI: 10.1021/ja104134e
• 发表时间: 2010
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Martin,Erik;Samoilova,RimmaI;Narasimhulu,KupalaV;Wraight,ColinA;Dikanov,SergeiA
• 通讯作者: Dikanov,SergeiA

Hydrogen bonding and spin density distribution in the Qb semiquinone of bacterial reaction centers and comparison with the Qa site.

细菌反应中心Qb半醌的氢键和自旋密度分布以及与Qa位点的比较。

• DOI: 10.1021/ja2001538
• 发表时间: 2011
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Martin,Erik;Samoilova,RimmaI;Narasimhulu,KupalaV;Lin,Tzu-Jen;O'Malley,PatrickJ;Wraight,ColinA;Dikanov,SergeiA
• 通讯作者: Dikanov,SergeiA

Hydrogen bonding between the Q(B) site ubisemiquinone and Ser-L223 in the bacterial reaction center: a combined spectroscopic and computational perspective.

细菌反应中心 Q(B) 位点泛半醌和 Ser-L223 之间的氢键：光谱和计算相结合的视角。

• DOI: 10.1021/bi300834w
• 发表时间: 2012
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Martin,Erik;Baldansuren,Amgalanbaatar;Lin,Tzu-Jen;Samoilova,RimmaI;Wraight,ColinA;Dikanov,SergeiA;O'Malley,PatrickJ
• 通讯作者: O'Malley,PatrickJ