ROOT CELL SHAPE DETERMINATION IN ARABIDOPSIS THALIANA

这里的重点是乙烯不敏感，根特异性突变体，影响 根系生长突变体以下列方式分离。 1-氨基环丙烷-1-羧酸（ACC），乙烯的前体，在5 X 10 exp-7 M通过降低根细胞长度抑制野生型根生长。突变体 没有显示这种减少是孤立的。在这些变种人中， 影响植物对乙烯的反应。到目前为止，一个突变体leir 10 已经被详细研究过了有五个目标： 1)确定leir 10/ere和leir 10/mre双突变体的表型。突变体 这些类别在标准条件下具有受损的根生长。抑制 乙烯合成的减少减轻了突变体的表型。这些上位性研究 得到leir 10是否参与乙烯合成的问题， perception. 2.）通过RT-PCR测量相关克隆基因的转录水平 在野生型和leir的乙烯合成或感知中10。3.）第三章筛选 leir的第二位点抑制子和增强子10 4.）继续初步 其他假定的乙烯不敏感的根特异性突变体的研究。5.） 筛选额外的T-DNA群体以获得额外的乙烯不敏感性， 根特异突变体

The focus here is on ethylene-insensitive, root-specific mutants that affect root growth. Mutants are isolated in the following way. 1-aminocyclopropane-1-carboxylic acid (ACC) a precursor of ethylene, at 5 X 10exp-7 M inhibits wildtype root growth by decreasing root cell length. Mutants not showing this reduction have been isolated. Among these mutants, those affecting plant response to ethylene are discarded. So far, one mutant, leir10 has been studied in some detail. There are five objectives: 1) determine phenotype of leir10/ere and leir10/mre double mutants. Mutants in these classes have impaired root growth under standard conditions. Inhibition of ethylene synthesis relieves the mutant phenotype. These epistatic studies get at the question of whether leir10 is involved in ethylene synthesis or perception. 2.) Measure, via RT-PCR transcript levels of cloned genes involved in ethylene synthesis or perception in wildtype and leir10. 3.) Screen for second-site suppressors and enhancers of leir10 4.) Continue preliminary studies of other putative ethylene-insensitive, root-specific mutants. 5.) Screen additional T-DNA populations for additional ethylene-insensitive, root-specific mutants