STRUCTURAL ANALYSIS OF THE GENOME OF RESPIRATORY SYNCYTIAL VIRUS

呼吸道合胞病毒基因组的结构分析

• 批准号: 6288840
• 负责人: PETER LEON COLLINS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6288840
• 关键词: capsid; chloramphenicol acetyltransferase; gene induction /repression; genetic regulatory element; genome; luciferin monooxygenase; microorganism culture; molecular cloning; recombinant virus; replicon; respiratory syncytial virus; structural biology; tissue /cell culture; transfection; virus genetics; virus replication

Respiratory syncytial virus (RSV) is an enveloped RNA virus that is the most important viral agent of pediatric respiratory tract disease worldwide. It currently lacks a licensed vaccine. RSV is a member of the paramyxovirus family of Order Mononegavirales, the nonsegmented negative strand RNA viruses, and RSV is the most complex member of the group. This large group of viruses includes important pathogens such as measles, mumps, rabies and Ebola viruses. Knowledge of the organization and expression of the RSV genome would help guide efforts to make attenuated recombinant viruses as vaccines, which is the major goal of related projects in this laboratory. We previously sequenced the 15,222-nucleotide genome of RSV, mapped its genes and its transcription signals, and analyzed its mechanism of gene expression. We currently are performing detailed mutagenic and functional analysis of the promoters and encapsidation signals at the 3 end of genomic and antigenomic (replicative intermediate) RNA. - Virus, vaccine, live- attenuated viral vaccine, pediatrics, infectious disease, respiratory tract disease, recombinant DNA

呼吸道合胞病毒 (RSV) 是一种有包膜 RNA 病毒，是全世界儿科呼吸道疾病最重要的病毒病原体。目前缺乏获得许可的疫苗。 RSV 是单链病毒目副粘病毒科的成员，是一种不分节段负链 RNA 病毒，RSV 是该组中最复杂的成员。这一大类病毒包括麻疹、腮腺炎、狂犬病和埃博拉病毒等重要病原体。了解 RSV 基因组的组织和表达将有助于指导减毒重组病毒疫苗的研发，这是本实验室相关项目的主要目标。我们之前对RSV的15,222个核苷酸的基因组进行了测序，绘制了其基因和转录信号图谱，并分析了其基因表达机制。我们目前正在对基因组和反基因组（复制中间体）RNA 3 端的启动子和衣壳化信号进行详细的诱变和功能分析。 - 病毒、疫苗、减毒活病毒疫苗、儿科、传染病、呼吸道疾病、重组DNA