QUANTITATIVE DETECTION OF CYTOMEGALOVIRUS BY USING A COMMERCIALLY DEVELOPED RESEA

巨细胞病毒(CMV)对接受异基因骨髓移植的患者可能是毁灭性的。在这些患者中，快速检测CMV感染可以挽救生命，使及时给予抗CMV药物。临床上还需要对临床患者中存在的巨细胞病毒进行定量测量，以监测治疗和预测耐药变异的出现。一家商业供应商已经开发出一种研究分析方法，允许使用定量聚合酶链式反应(PCR)来测量从全血中提取的CMV DNA。我们将用美国医学实验室采集的外周血白细胞对该方法进行评价，并将其结果与美国医学实验室进行的检测多形核白细胞和单核细胞中存在CMV pp65早期结构蛋白的CMV抗原血症定量分析结果进行比较。

Cytomegalovirus (CMV) can be devastating in the patient who has undergone an allogeneic bone marrow transplant. Rapid detection of CMV infection in these patients can be life-saving, allowing the prompt administration of anti-CMV drugs. There is also a clinical need for a quantitative measure of the CMV present in the clinical speci-men to allow for monitoring therapy and predicting emergence of drug-resistant variants. A research assay has been developed by a commercial vendor allowing the use of quanti-tative polymerase chain reaction (PCR) to measure the CMV DNA extracted from whole blood. We will evaluate this PCR assay by using peripheral blood leukocytes collected by American Medical Laboratories and compare the results with the CMV antigenemia quan-titative assay (performed at American Medical Laboratories) that detects the presence of CMV pp65 early structural protein in polymorphonuclear leukocytes and monocytes.