High-Density Growth of 96 and 384 Bacterial Samples

描述（由申请人提供）：在第一阶段，我们将开发一个原型， 一个简单的，具有成本效益的设备，允许细菌生长在任何类型的96 或384孔微量滴定板或深孔块，而不使用昂贵的 设备.该产品将简化细菌生长在密集的空间 微量滴定板，但仍然导致稳健的培养，DNA产量更大 比在锥形瓶或更大的振荡器试管中的等效体积生长更大。在 第一阶段，我们将进一步开发和优化曝气/搅拌原型 在第一阶段之前成功实验的设备。这个原型 使用受控的预热、无菌、加湿的空气流， 通过培养基同时完成通气、加热和 搅拌紧密堆积的培养物。原型与小型 和从50 μ l至2.0 ml的大培养体积，因此可以使用 用于在96或384深孔板中生长。培养体积的DNA产量为 低至300 μ l的量足以进行自动DNA测序。的 拟议的产品将是廉价的建设，并将在一个 完全独立、紧凑的方式，无需外部培养箱， 振荡器、搅拌器或氧气源，如与其它装置一起使用， 这个任务。我们相信该设备可以以低于2000美元的价格出售。 该产品将大大提高从细胞中生长和制备DNA的能力， 重组培养，从而提高质量和通过把大 规模DNA测序和分析工作。 拟议商业应用：不可用

DESCRIPTION (provided by applicant): In Phase I, we will develop a prototype of a simple, cost-effective device that allows bacterial growth in any type of 96 or 384 well microtiter plates or deep well blocks without the use of expensive equipment. The product will simplify bacterial growth in tightly spaced microtiter plates, yet still result in robust culture with DNA yields greater than equivalent volume growth in Erlenmeyer or larger shaker test tubes. In phase I, we will further develop and optimize an aeration/agitation prototype device that was successfully experimented with prior to Phase I. This prototype uses a controlled stream of pre-heated, sterile, humidified air that is bubbled through the culture medium to simultaneously accomplish aeration, heating and agitation of the tightly packed cultures. The prototype works well with small and large culture volumes ranging from 50 ul to 2.0 ml, and thus could be used for growth in 96 or 384 deep well plates. The DNA yield from culture volumes as low as 300 ul was sufficient quantity to perform automated DNA sequencing. The proposed product will be inexpensive to construct and will operate in a completely self-contained, compact manner without an external incubator, shaker, stirrer or oxygen source as are used with other devices that accomplish this task. We believe that the device can be sold for less than $2000.The product will greatly enhance the ability to grow and prepare DNA from recombinant culture, thereby improving the quality and through put of large scale DNA sequencing and analysis efforts. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE