HIGH-THROUGHPUT CRYSTALLIZATION ROBOT

高通量结晶机器人

• 批准号: 6213594
• 负责人: ENRIQUE E ABOLA
• 金额: $ 10万
• 依托单位: SYRRX, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6213594
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; computer program /software; crystallization; high throughput technology; protein structure; protein structure function; proteomics; robotics; technology /technique development

DESCRIPTION: (Adapted from the applicant's abstract) It is the mission of the Protein Structure Initiative to develop an understanding of gene products via structural studies. The size and complexity of genomes precludes the use of standard laboratory techniques if this goal is to be achieved within a single human lifetime. Development of technology and new laboratory protocols are therefore needed. The focus of our R&D is the development and commercialization of "enabling technologies" using the high-throughput paradigm that has been successfully applied to the Human Genome Project, and to a lesser extent, combinatorial chemistry. A well-known bottleneck in the process of determining macromolecular structures is the generation of crystalline samples. We are seeking funds to design and construct a high throughput crystallization robot capable of handling greater than 10,000 molecular targets per year. Achieving this goal will require the development of new protocols such as handling of very small sample volumes, the creation of new crystallization screens (including the extension of existing 3-component screens), and the development of imaging systems capable of recognizing successful experiments with minimal human intervention. This Phase I proposal seeks funds to conduct feasibility studies to generate engineering drawings, to conduct crystallization studies on an existing robot prototype, and to populate and analyze a crystallization database. In Phase II the high-throughput crystallization robot will be constructed and a crystallization database will be built. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：(改编自申请者的摘要)这是 蛋白质结构倡议通过以下途径发展对基因产品的理解 结构研究。基因组的大小和复杂性排除了使用 标准实验室技术如果要在一个单一的 人的一生。技术的发展和新的实验室方案 因此需要。我们的研发重点是产品的开发和商业化 使用高吞吐量范例的“使能技术” 成功地应用于人类基因组计划，在较小的程度上， 组合化学。在确定的过程中一个众所周知的瓶颈 大分子结构是晶体样品的生成。我们是 寻求资金设计和建造高通量结晶机器人 每年能够处理超过10,000个分子靶标。实现 这一目标将需要开发新的协议，例如处理 非常小的样品体积，创造出新的结晶屏幕 (包括对现有三分量屏幕的扩展)，以及开发 成像系统能够以最低限度识别成功的实验 人为干预。这一阶段的提案寻求资金进行可行性 研究生成工程图纸，进行结晶研究 现有的机器人原型，并填充和分析结晶 数据库。在第二阶段，高通量结晶机器人将 并建立结晶数据库。 建议的商业应用：不可用