New Class of Long Wavelength Fluorescent Laser Dyes

新型长波长荧光激光染料

• 批准号: 6337220
• 负责人: Nicholas Cairns
• 金额: $ 10万
• 依托单位: COMBINIX, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6337220
• 关键词: chemical property; chemical structure function; chemical synthesis; combinatorial chemistry; fluorescence polarization; fluorescent dye /probe; high performance liquid chromatography; infrared radiation; mass spectrometry; technology /technique development

DESCRIPTION: (Applicant's abstract) Sources of commercially available dyes that fluoresce in the long visible/near infrared (NIR) spectrum between 650-900nm, containing a variety of reactive chemical handles are severely limited and available dyes and prohibitively expensive for many research establishments. This project seeks to introduce a new class of long wavelength/ near infrared (NIR) fluorescent dyes. Important applications of these compounds include fluorescence polarization (FP) assays and techniques, near infrared optical imaging of animal tissue, conventional fluorescence and enzymatic fluorescence assays. Initially, a combinatorial chemistry array of 27 compounds will be synthesized on a small scale (100-200ug) characterized by HPLC and Mass Spectrometry, and tested for their fluorescence and polarization properties and chemical / photostability. A smaller number of the most promising candidates (3-5) will then be synthesized on a larger scale (20-50mg) to allow for chemical activation of the probes (e.g. by the introduction of an amine-reactive N-hydroxysuccinimidyl ester handle) and for full structural characterization including NMR and elemental analysis studies. Two or three selected compounds will then be tested in a model fluorescence polarization haloperidol immunoassay. The best compound(s) will then be fitted with a variety of chemical handles for covalent attachment to various functional groups. PROPOSED COMMERCIAL APPLICATION: There is a real shortage of long-wavelength / near infrared fluorescent dyes in the 700-900nm region with chemically reactive handles commercially available at the present time despite large increasing demands for such compounds. This new class of long wavelength / near infrared dyes will be designed to bridge this gap in the market. The dyes will have good chemical and photostability, excellent fluorescence properties and water solubility, and low non-specific binding to proteinacious material and nucleic acids.

描述：（申请人的摘要）市售染料的来源， 在650- 900 nm之间的长可见/近红外（NIR）光谱中发荧光， 含有多种反应性化学手柄的材料受到严重限制， 可获得的染料，并且对于许多研究机构来说过于昂贵。 该项目旨在引入一类新的长波长/近红外 (NIR)荧光染料。这些化合物的重要应用包括 荧光偏振（FP）分析和技术、近红外光学 动物组织成像、常规荧光和酶荧光 测定。最初，27种化合物的组合化学阵列将被 在小规模（100- 200 μ g）上合成，通过HPLC和质谱表征 光谱，并测试其荧光和偏振特性， 化学/光稳定性。少数最有希望的候选人 (3-5)然后将以更大的规模（20- 50 mg）合成，以允许 探针的化学活化（例如通过引入 胺反应性N-羟基琥珀酰亚胺基酯柄）和用于全结构 表征包括NMR和元素分析研究。两三 然后将在模型荧光偏振中测试选定的化合物 氟哌啶醇免疫测定最佳组合将配备一个 用于共价连接到各种官能化的 组 拟定商业应用： 在700- 900 nm范围内的长波长/近红外荧光染料存在真实的短缺 目前市场上可买到的具有化学反应性手柄的区域， 对这些化合物的需求不断增加。 这类新的长波长/近 红外线染料将被设计来填补市场上的这一空白。 染料会有很好的 化学和光稳定性，优异的荧光性能和水溶性， 与蛋白质物质和核酸的非特异性结合。