Novel Extended Red-Shifted Xanthene Dyes

新型扩展红移呫吨染料

• 批准号: 6689085
• 负责人: Nicholas Cairns
• 金额: $ 12.34万
• 依托单位: COMBINIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6689085
• 关键词: animal tissue; benzopyrans; bioimaging /biomedical imaging; chemical synthesis; electrospray ionization mass spectrometry; enzyme inhibitors; fluorescence resonance energy transfer; fluorescent dye /probe; infrared radiation; infrared spectrometry; interferometry; nuclear magnetic resonance spectroscopy; protein kinase C; technology /technique development

DESCRIPTION (provided by applicant): Fluorescent dyes that operate in the long visible / near infra-red (NIR) region of the electromagnetic spectrum are excellent reporter components of in vivo targeting molecular probes, fluorescent ligands, enzyme substrates and general tracers. Such dyes are in very short supply at the present time and those that are commercially available are very expensive or are only available under severely restrictive license agreements. Many of the available dyes have undesirable properties such as relatively high non-specific binding and a tendency to aggregate and are prohibitively expensive due in part to unoptimized synthetic methods and inadequate molecular design. The main focus of this work is to discover novel, long wavelength near-infrared dyes that are designed for use in biomedical in vivo imaging, high throughput screening and other applicable techniques such as immunofluorescence microscopy, fluorescence resonance energy transfer and fluorescence activated cell sorting and counting. Developments in imaging technologies have had a profound impact on clinical medicine including, ultrasound scanning, magnetic resonance imaging, x-ray computed tomography and nuclear tomography imaging. These systems are primarily used for displaying anatomical, physiological and metabolic parameters but they are increasingly being used in experimental animal systems for imaging at the cellular and molecular levels in vivo. These currently used imaging systems rely on physical parameters and properties to generate image contrast such as, sound impedance, electromagnetic wave impedance or nuclear alignments. In vivo fluorescence imaging offers more sensitive early stage detection of tumors that is very difficult to achieve with existing imaging techniques. Novel synthetic chemistry pathways will be developed that will allow gram-scale synthesis of the dyes in high purity and will result in products that will be more widely available to the research and scientific community.

描述（由申请人提供）：在电磁波谱的长可见/近红外（NIR）区域工作的荧光染料是体内靶向分子探针、荧光配体、酶底物和一般示踪剂的优良报告组分。这种染料目前供应非常短缺，并且那些可商购的染料非常昂贵或仅在严格限制的许可协议下才可获得。许多可用的染料具有不期望的性质，例如相对高的非特异性结合和聚集的倾向，并且部分由于未优化的合成方法和不充分的分子设计而过于昂贵。这项工作的主要重点是发现新的，长波长的近红外染料，设计用于生物医学在体内成像，高通量筛选和其他适用的技术，如免疫荧光显微镜，荧光共振能量转移和荧光激活细胞分选和计数。成像技术的发展对临床医学产生了深远的影响，包括超声扫描、磁共振成像、X射线计算机断层扫描和核断层扫描成像。这些系统主要用于显示解剖学、生理学和代谢参数，但它们越来越多地用于实验动物系统中，用于在体内细胞和分子水平上成像。这些当前使用的成像系统依赖于物理参数和性质来生成图像对比度，诸如声阻抗、电磁波阻抗或核对准。体内荧光成像提供了更灵敏的肿瘤早期检测，这是现有成像技术难以实现的。将开发新的合成化学途径，使高纯度染料的克级合成成为可能，并将导致研究和科学界更广泛地获得产品。