Engineered Antibodies for Imaging Gene Expression

用于基因表达成像的工程化抗体

• 批准号: 6337628
• 负责人: A J CHMURA
• 金额: $ 9.78万
• 依托单位: LEXRITE LABS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2001-10-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6337628
• 关键词: CHO cells; antibody; bioengineering /biomedical engineering; bioimaging /biomedical imaging; cell membrane; chelating agents; enzyme linked immunosorbent assay; gene expression; gene induction /repression; gene targeting; immunologic substance development /preparation; membrane proteins; metal complex; metals; nucleic acid sequence; polymerase chain reaction; positron emission tomography; radiotracer; receptor binding; receptor expression; tissue /cell culture; transfection; western blottings

DESCRIPTION (Provided by Applicant): Observing the expression of transfected genes in a living system, with minimal disturbance, is important for the future development of gene therapy. We propose an approach that possesses many of the best features of recent advances in this area, but extends the power of imaging in new directions. The metallic elements possess a variety of useful physical properties that make them valuable as probes. These include radioactive emissions of photons for gamma or PET imaging, radioactive emissions of particles for therapy, paramagnetism for MRI contrast, luminescence for optical imaging, etc. Chemistry for making use of these properties in biomedical applications is fundamental to the further development of imaging instrumentation and technology. We will demonstrate the feasibility of imaging gene expression with metal chelates by engineering an antibody so that it is expressed as a cell-surface receptor for metal chelates, under the control of an inducible transcription unit. When expressed on the cell surface, this new receptor will capture labeled chelates and ultimately provide an image of the cluster of transformed cells that express the receptor. Phase 2 will focus on development of new products that apply this technology to a useful set of metallic elements and imaging techniques. PROPOSED COMMERCIAL APPLICATION: High-resolution imaging of gene expression in cancer cells is important to the future science and technology of cancer treatment. The molecular tools that we prepare will allow the physical properties of metallic elements to be employed in new ways. They will find uses in a variety of high-technology imaging applications, in both research and (ultimately) clinical applications.

描述（由申请人提供）：观察转染的 基因在一个生命系统中，以最小的干扰，是重要的未来， 基因治疗的发展。我们提出了一种方法，拥有许多 最好的特点，最新的进展，在这一领域，但扩大了权力的成像 新方向金属元素具有多种有用的物理性质， 这些特性使它们成为有价值的探针。其中包括放射性 用于伽马或PET成像的光子发射， 用于治疗的粒子，用于MRI对比的顺磁性，用于光学的发光 成像等。化学利用这些性质在生物医学 应用是成像技术进一步发展的基础 仪器和技术。我们将证明成像的可行性 基因表达与金属螯合物通过工程抗体，使其 表达为金属螯合物的细胞表面受体，受 一个诱导转录单位当在细胞表面表达时， 受体将捕获标记的螯合物，并最终提供图像的 表达受体的转化细胞簇。第二阶段将侧重于 开发新产品，将这项技术应用于一系列有用的 金属元素和成像技术。 拟定商业应用： 癌细胞基因表达的高分辨率成像对未来科学很重要 和癌症治疗技术。 我们准备的分子工具将允许 金属元素的物理性质将以新的方式使用。 他们会发现 在各种高科技成像应用中，在研究和（最终） 临床应用。