REGULATION OF PROGESTERONE BIOSYNTHESIS BY THE CORPUS LUTEUM

黄体合成并分泌类固醇激素 孕酮是建立和维持 怀孕 黄体和孕酮的分泌也 调节女性月经周期的持续时间和 家畜 对黄体的操纵导致了 妇女避孕药具和同步发情周期的方法， 家畜 然而，高胚胎死亡率（大约 50%的妇女，16-42%的牲畜）和目前的不良副作用 一些妇女的避孕药决定了新的方法来管理语料库 黄体进行研究。 多胺调节细胞生长和细胞内 功能协调发展的 但多胺的作用与黄体功能有一定关系 没有被研究过。 拟议的研究将审查以下方面的作用： 多胺与孕酮合成及mRNA表达 编码黄体内的类固醇生成酶。 细胞培养 分子生物学技术将被用来完成这些 目的是利用母羊，这是由许多研究人员使用， 人类和其他家畜的动物模型。 黄体 将从发情周期第10天的母羊中获得。 黄体组织 将被酶消化以产生单细胞悬浮液。 培养的细胞将用多胺和孕酮分泌处理 将通过RIA进行测量。 将收获培养的细胞，并将mRNA 提取。 将通过以下方法分析编码类固醇生成酶的mRNA： 北方印迹杂交方法。 学生将获得全面的 所需的细胞/组织培养和分子生物学技术培训 在许多科学领域具有竞争力。

The corpus luteum synthesizes and secretes the steroid hormone progesterone which is required for the establishment and maintenance of pregnancy. The corpus luteum and the secretion of progesterone also regulates the duration of menstrual cycles in women and estrous cycles in domestic animals. Manipulation of the corpus luteum has resulted in contraceptives for women and means of synchronizing estrous cycles in domestic animals. Yet the high rate of embryonic mortality (approximately 50% in women, 16-42% in livestock) and undesirable side effects of current contraceptives for some women dictates new methods to govern the corpus luteum be investigated. Polyamines regulate cell growth and intracellular functions. However, the role of polyamines and corpus luteum function has not been studied. The research proposed will examine the role of polyamines and the synthesis of progesterone and expression of mRNA encoding the steroidogenic enzymes within the corpus luteum. Cell culture and molecular biology techniques will be used to accomplish these objectives utilizing ewes, which are used by numerous researchers as animal models for both humans and other domestic animals. Corpora lutea will be obtained from ewes on day 10 of the estrous cycle. Luteal tissue will be enzymatically digested to yield single cell suspensions. Cultured cells will be treated with polyamines and progesterone secretion will be measured by RIA. Cultured cells will be harvested and mRNA extracted. mRNA encoding the steroidogenic enzymes will be analyzed by Northern blot hybridization methods. Students will receive comprehensive training in cell/tissue culture and molecular biology techniques required to be competitive in numerous scientific fields.