RELIABILITY/REPRODUCIBILITY OF MEASUREMENT OF CTL ACTIVITY IN HIV

HIV 中 CTL 活性测量的可靠性/可重复性

• 批准号: 6297570
• 负责人: ROBERT C. BOLLINGER
• 金额: $ 0.23万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6297570
• 关键词: AIDS; HIV infections; clinical research; cytotoxic T lymphocyte; flow cytometry; human subject; leukocyte activation /transformation; lymphocyte proliferation; virus load

This is a study to assess the reliability and reproducibility of measurements of CTL activity and HIV-specific proliferation in a blinded study of 15 HIV infected and 3 HIV uninfected control individuals. Proof of blood mononuclear cells were collected at time 0 and two weeks later and CTL assays of HIV specific activity as well as HIV specific proliferative activity were assessed in Dr. Bollinger and Dr. Lederman's Lab. All 18 of the proposed subjects were fully enrolled and accrued for this study. In addition, HIV plasma viral load and cell surface flow cytometric analysis was performed in Dr. Quinn's lab on all samples. Preliminary results of this study are outlined in an abstract recently submitted to the 121 World AIDS Conference in Geneva scheduled for June, 1998. In summary, in this blinded analysis HIV-specific CTL activity directed against multiple HIV antigens measured by bulk culture antigen specific stimulation of frozen PBMC's demonstrated statistically significant correlation with viral load and CD4 count. The best method for correlating HIV specific CTL with clinical stage utilized an area under specific lysis curve analysis. The conclusion was that this method may be useful for measuring CTL activities in clinical trials.

这是一项评估可靠性和再现性的研究 在盲法中测量 CTL 活性和 HIV 特异性增殖 对 15 名 HIV 感染者和 3 名未感染 HIV 的对照个体进行的研究。 在第 0 周和第 2 周收集了血液单核细胞的证据 随后进行 HIV 特异性活性和 HIV 特异性的 CTL 检测 Bollinger 博士和 Lederman 博士的增殖活性评估 实验室。 所有 18 个拟议科目均已全部注册并累积 对于这项研究。 此外，HIV血浆病毒载量和细胞表面 Quinn 博士的实验室对所有样本进行了流式细胞术分析 样品。 摘要概述了这项研究的初步结果 最近提交给第121届日内瓦世界艾滋病大会预定 1998 年 6 月。总而言之，在这项盲法分析中，HIV 特异性 CTL 通过批量培养测量针对多种 HIV 抗原的活性 统计证明冷冻 PBMC 的抗原特异性刺激 与病毒载量和CD4计数显着相关。 最好的方法 用于将 HIV 特异性 CTL 与临床分期相关联的领域 根据特定的裂解曲线分析。 结论是，这 方法可能有助于测量临床试验中的 CTL 活性。