RELIABILITY/REPRODUCIBILITY OF MEASUREMENT OF CTL ACTIVITY IN HIV

HIV 中 CTL 活性测量的可靠性/可重复性

• 批准号: 6114405
• 负责人: ROBERT C. BOLLINGER
• 金额: $ 2.06万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6114405
• 关键词: AIDS; HIV infections; clinical research; cytotoxic T lymphocyte; flow cytometry; human subject; leukocyte activation /transformation; lymphocyte proliferation; virus load

This is a study to assess the reliability and reproducibility of measurements of CTL activity and HIV-specific proliferation in a blinded study of 15 HIV infected and 3 HIV uninfected control individuals. Proof of blood mononuclear cells were collected at time 0 and two weeks later and CTL assays of HIV specific activity as well as HIV specific proliferative activity were assessed in Dr. Bollinger and Dr. Lederman's Lab. All 18 of the proposed subjects were fully enrolled and accrued for this study. In addition, HIV plasma viral load and cell surface flow cytometric analysis was performed in Dr. Quinn's lab on all samples. Preliminary results of this study are outlined in an abstract recently submitted to the 121 World AIDS Conference in Geneva scheduled for June, 1998. In summary, in this blinded analysis HIV-specific CTL activity directed against multiple HIV antigens measured by bulk culture antigen specific stimulation of frozen PBMC's demonstrated statistically significant correlation with viral load and CD4 count. The best method for correlating HIV specific CTL with clinical stage utilized an area under specific lysis curve analysis. The conclusion was that this method may be useful for measuring CTL activities in clinical trials.

这是一项研究，以评估的可靠性和再现性， 在一个盲法的实验中， 15例HIV感染者和3例HIV未感染者对照研究。 在时间0和两周收集血液单核细胞的证明 HIV特异性活性以及HIV特异性 增殖活性在Bollinger博士和Lederman博士的 实验室 所有18例拟定受试者均已完全入组并累积 用于本研究。 此外，HIV血浆病毒载量和细胞表面 在Quinn博士的实验室对所有 样品 本研究的初步结果在摘要中概述 最近提交给定于日内瓦举行的第121届世界艾滋病大会的 1998年6月。 总之，在这项盲法分析中，HIV特异性CTL 通过大量培养测量的针对多种HIV抗原的活性 冷冻PBMC的抗原特异性刺激在统计学上证明 与病毒载量和CD4计数显著相关。 最好的方法 为了将HIV特异性CTL与临床分期相关联， 特异性裂解曲线分析。 结论是， 该方法可用于临床试验中CTL活性的测定。