CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

• 批准号: 6316510
• 负责人: Mark A Ritter
• 金额: $ 28.96万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-30 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316510
• 关键词: animal tissue; biomedical equipment resource; biomedical facility; calcium flux; cell cycle; experimental designs; flow cytometry; human tissue

The Flow Cytometry Laboratory is located in a 400 square foot laboratory, K4/535, with an adjacent office, K4/563D. Analyses requiring both single and dual laser excitation and cell sorting services are offered to investigators. A wide range of cytometric tools are available including measurements of cell size, cell cycle, intracellular CA++ and pH, and cell kinetics, as well as of standard surface and intracellular phenotypes. In addition to maintaining and assisting in the operation of equipment, the staff are involved in the development of new protocols and methodology and assist investigators in specific experimental design. Technical assistance on sample preparation, the operation of the instruments, data analysis, and interpretation is available on an individual basis. All facility computers are connected via the University network, including those available for off-line data analysis.

流式细胞仪实验室位于一个400平方英尺的实验室中， K4/535，设有邻近办公室，K4/563d。分析都需要两个 并提供双激光激发和细胞分类服务 调查人员。提供了各种各样的细胞仪工具，包括 细胞大小，细胞周期，细胞内Ca ++和pH的测量以及细胞 动力学以及标准表面和细胞内表型。在 除了维护和协助设备操作， 员工参与了新协议和方法论的制定， 协助研究人员进行特定的实验设计。技术援助 在样品准备，仪器的运行，数据分析， 并且可以单独使用解释。所有设施 计算机通过大学网络连接，包括 可用于离线数据分析。