Flow Cytometry

自1988年以来，流式细胞仪设备一直在支持UWCCC调查人员。它包括两个实验室 网站。主实验室位于临床科学中心(CSC)，为卫星改建 位于McArdle实验室中央校区的实验室将于秋季完工 2006年。该设施的工作人员从周一到周五的上午8：00到下午5：00，但技术是 训练有素的用户可随时使用。实验室工作人员维护所有仪器并提供支持 数据采集；分类；执行数据分析的说明；仪器操作说明， 关于实验设计、样品处理和染色方案的咨询.基本原理教育 通过研讨会、实验室会议或课程作业，以及一系列的三次讲座 每月提供初学者；现场染色设备；样品制备和样品分析 从临床试验中提交。这项服务也适用于没有研究实验室的临床医生 进行临床试验。(该机构致力于研究，不进行常规临床分析 用于患者诊断和治疗。) 该设施管理和操作以下设备：两台高速分拣机、FACScan、 FACSCalibur，LSR，Path Biolmager，用于数据分析的离线计算机，以及奥林巴斯荧光仪 显微镜。通过癌症中心支持赠款的支持，以及正在进行的重大 由癌症中心用机构基金和慈善基金投资，以及来自 成功的仪器设备赠款，该设施为UWCCC提供最新的尖端技术 会员。

The Flow Cytometry Facility has supported UWCCC investigators since 1988. It includes laboratories at two sites. The main laboratory is located at the Clinical Science Center (CSC) and remodeling for a satellite laboratory that will be located on the central campus at the McArdle Laboratory will be completed in Fall 2006. The Facility is staffed from 8:00 AM until 5:00 PM Monday through Friday, but the technologies are available to trained users at all times. The laboratory staff maintain all instruments and provide support for data acquisition; sorting; instruction in performing data analysis, instruction on instrument operation, consultation on experimental design, sample handling, and staining protocols; education on basic principles of flow cytometry through seminars, lab meetings, or course work, as well as a series of three lectures for beginners offered monthly; on-site staining facilities; and sample preparation and analysis of specimens submitted from clinical trials. This service is also available to clinicians without research laboratories who are conducting clinical trials. (The facility is dedicated to research and does not perform routine clinical analyses used in patient diagnosis and treatment.) The Facility manages and operates the following equipment: two high-speed sorters, FACScan, FACSCalibur, LSR, Pathway Biolmager, off-line computers for data analysis, and an Olympus Fluorescence Microscope. Through support of the Cancer Center Support Grant coupled with a significant on-going investment by the Cancer Center with institutional funds and philanthropic funds, as well as support from successful instrumentation grants, this facility provides the latest cutting-edge technology to UWCCC members.