CORE--NUCLEIC ACID FACILITY

核心--核酸设施

• 批准号: 6347274
• 负责人: MARSHA L. FRAZIER
• 金额: $ 25.41万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-30 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6347274
• 关键词: DNA; biomedical facility; cell line; genetic screening; genetic susceptibility; genetically modified animals; genotype; human genetic material tag; human tissue; laboratory mouse; neoplasm /cancer genetics; nucleic acid structure; oncogenes

The Nucleic Acid Core Facility is located in the Department of Gastrointestinal Oncology and Digestive Diseases in a 500 feet/2 laboratory. The Facility was created in the fall of 1996 through a CCSG supplement in Human Cancer Genetics. The NACF is a centralized facility for extraction of high-quality DNA from peripheral blood leukocytes and cell lines by using an Applied Biosystems Model 341 Nucleic Acid Extractor. This allows for the rapid isolation of highly purified, high- molecular-weight DNA. M.D. Anderson Cancer Center has a wealth of patient and family resources for studies of cancer-susceptibility genes. Most DNA isolation techniques used by individual laboratories required significant technical support and take 3 or more days. A centralized automated facility capable of producing PCR-grade DNA overnight from peripheral blood or cell is a major improvement in efficiency. The facility will be expanded to provide PCR-based genetic testing of transgenic animals. Because the high degree of product purity provided by the Model 341 Nucleic Acid Extractor will not be necessary for this testing, a more crude but rapid extraction procedure will be used to isolate DNA from mouse tails. The DNA will be subjected to PCR by using primates specifically designed to detect the transgenes, and animals positive for the transgenes will be identified. A large number of transgenic animal lines from over 20 different laboratories at M.D. Anderson are currently under investigation. Conducting DNA extraction from transgenic animal tails and PCR-based genetic testing in one centralized facility will be more efficient than performing these tasks in individual laboratories, as it will allow automation of each step by the use of Beckman Biomek 2000 robotic arms and multiplex PCR.

核酸核心设施位于 500英尺/2高原的胃肠道肿瘤学和消化系统疾病 实验室。该机制是在1996年秋季通过CCSG创建的 人类癌症遗传学补充剂。NACF是一个集中式设施 用于从外周血白细胞中提取高质量的DNA和 应用生物系统模型341核酸建立细胞系 萃取器。这使得能够快速分离出高纯度、高纯度的 分子量DNA。M.D.安德森癌症中心有大量的病人 以及用于癌症易感基因研究的家庭资源。大多数DNA 个别实验室使用的隔离技术需要大量的 技术支持，并需要3天或更长时间。集中式自动化 一种能够连夜从外周生产PCR级DNA的设备 血液或细胞是效率的重大改进。该设施将是 扩大到提供基于聚合酶链式反应的转基因动物基因检测。 因为341型号所提供的高度产品纯度 这次检测将不需要核酸萃取器，更多的是 将使用粗略但快速的提取程序从 老鼠尾巴。DNA将通过使用灵长类动物进行聚合酶链式反应 专门为检测转基因而设计的，动物对转基因呈阳性 转基因将被识别出来。大量的转基因动物 来自M.D.安德森的20多个不同实验室的生产线目前 正在调查中。从转基因动物中提取DNA 将在一个集中的设施中进行基于Tail和PCR的基因检测 比在单个实验室中执行这些任务更高效，因为 它将通过使用Beckman Biomek 2000实现每一步的自动化 机械臂和多重聚合酶链式反应。