Biospecimen Extraction

生物样本提取

• 批准号: 7695930
• 负责人: MARSHA L. FRAZIER
• 金额: $ 5.26万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-28 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7695930
• 关键词: Alkaline Phosphatase; Arts; Base Sequence; Basic Science; Blood; Budgets; Cancer Center Support Grant; Complementary DNA; Consult; CpG Islands; DNA; DNA Library; Databases; Detection; Exodeoxyribonuclease I; Fees; Freezing; Funding; Funding Agency; Future; Genotype; Goals; Grant; Guidelines; Human; Laboratories; Leukocytes; Methylation; Nucleic Acids; Nucleic acid sequencing; Paraffin Embedding; Patients; Peer Review; Plasma; Polymerase Chain Reaction; Polymorphism Analysis; Preparation; Price; Procedures; Process; RNA; Recording of previous events; Research; Research Personnel; Resources; Saliva; Sampling; Sequence Analysis; Serum; Services; Shrimp; Single Nucleotide Polymorphism; Solid; Source; Technology; Tissues; University of Texas M D Anderson Cancer Center; Update; Whole Blood; base; cancer prevention; gel electrophoresis; improved; instrumentation; member; new technology; peripheral blood; programs; repository

The Biospecimen Extraction Resource (BER) is a centralized laboratory for standardized, high-quality, reasonably-priced DMA and RNA extraction from various sources. The BER performs PCR-based analysis of these nucleic acids including pyrosequencing for genotyping of single nucleotide polymorphisms and for detection of CpG island methylation. In addition, the BER consults with non-laboratory-based investigators to help them accomplish their laboratory-based goals related to the facility. The BER is located in the newlyconstructed Basic Science Research Building (BSRB). The BER uses state-of-the-art instrumentation including the Autopure LS automated DMA extraction machine and the PSQ pyrosequencer. The BER has an 8-member oversight committee. A website provides information about services, guidelines for preparing samples for DMA extraction, and other procedures performed by the facility, as well as forms to be completed when requesting service. In the current grant period, the BER was used by 35 different investigators from 17 programs with 98% of usage coming from investigators having peer-reviewed funding. Most of the DMA extractions are from human peripheral blood leukocytes or saliva. An increase of over 400% has been seen over the past year compared to those extracted in 2002. The source of funds for the proposed operating budget of the BER for year 33 (July 1, 2008-June 30, 2009) is 14% from TexGen, 53% from user fees, and 34% from the CCSG. Future plans include continued expansion to large projects such as TexGen, the "LBJ Patient History Database-Serum/DNA Bank" and the "Repository of Tissue and Blood on Patients in the Cancer Prevention Center."

生物标本提取资源（BER）是一个集中的实验室，用于标准化，高质量， 价格合理的DMA和RNA提取从各种来源。BER执行基于PCR的分析 包括用于单核苷酸多态性基因分型和用于单核苷酸多态性基因分型的焦磷酸测序。 CpG岛甲基化的检测。此外，BER还与非实验室研究人员进行协商， 帮助他们实现与设施相关的实验室目标。BER位于新建的 基础科学研究大楼（BSRB）。BER使用最先进的仪器 包括Autopure LS自动DMA提取机和PSQ热测序仪。BER已经 一个八人监督委员会一个网站提供有关服务的信息， 用于DMA提取的样品，以及机构执行的其他程序，以及 在请求服务时完成。在目前的赠款期间，BER被35个不同的 来自17个项目的研究人员，98%的使用来自具有同行评审资金的研究人员。 大多数DMA提取物来自人外周血白细胞或唾液。增长超 与2002年的开采量相比，去年的开采量增加了400%。的资金来源 BER第33年（2008年7月1日至2009年6月30日）的拟议运营预算为TexGen的14%， 来自用户费，34%来自CCSG。未来的计划包括继续扩大到大型项目， 作为TexGen，“LBJ患者历史数据库-血清/DNA库”和“组织和血液库 癌症预防中心的病人。"