CORE--HISTOLOGY AND IMMUNOHISTOCHEMISTRY

（申请人描述）在加州大学圣地亚哥分校，许多研究人员正在进行研究，需要对人类和小鼠肿瘤及其同源正常组织进行显微镜分析。其他人正在研究遗传和表观遗传干预在胚胎和成年动物中引起的变化的细节，以便了解调节正常和肿瘤发育的机制。所有这些研究人员研究与癌症问题相关的主题，都需要准备好，负担得起高质量的组织学和免疫组织化学技术服务，以及专家的组织病理学和胚胎学建议。这一资源已经为中心的成员提供了至关重要的服务，并且随着人们越来越广泛地认识到肿瘤病变及其前体的显微组织评估是癌症研究中良好可靠的研究技术的基石，它的重要性将会增加。它提供了一个给定样本的肿瘤性质和准确的病理分级和分期的确认。对于实验室数据与病变诊断类别的相关性而言，它是必不可少的，并且在生化或分子生物学分析开始之前，它还提供了样品中肿瘤组织存在的重要质量控制。此外，在这种实验中，建立被视为正常对照的组织中肿瘤的缺失是必不可少的。如果没有这些重要的信息，大量的时间、精力和金钱就会被浪费，结论也会变得毫无意义。组织学和免疫组织化学共享资源的目标是为同行评审的癌症中心成员和附属研究人员提供这些设施。该资源（以前称为组织库）在过去四年中已发展成为一个广泛使用的补充设施，并增加了工作人员。下一个资助周期的任务是与加州大学圣地亚哥分校nci资助的其他共享资源密切联系，特别是动物实验、信息学、分子病理学和转基因小鼠，以创建一个极好的互动小组，用于分析人体组织样本、携带肿瘤的实验动物、携带转基因的实验动物和“敲除”小鼠。

Description: (Applicant's Description) At UCSD many investigators are now conducting research which requires the microscopical analysis of human and murine tumors and their cognate normal tissues. Others are examining the details of changes induced in embryonic and adult animals by genetic and epigenetic intervention, so as to understand the mechanisms regulating normal and neoplastic development. All of these research workers studying topics relevant to the cancer problem need ready, affordable access to high quality histological and immunohistochemical technical services and to expert histopathological and embryological advice. This resource already provides pivotally important services to the Members of the Center and its importance will grow as it becomes more widely appreciated that microscopical, tissue-based evaluation of neoplastic lesions and their precursors is the cornerstone of good reliable research technique in cancer research. It provides confirmation of the neopla stic nature of a given sample and accurate pathological grading and staging. For the correlation of laboratory data with the diagnostic category of a lesion, it is indispensable and it also provides vital quality control of the presence of tumor tissue in a sample before biochemical or molecular biological analysis begins. Furthermore, it is essential for establishing the absence of tumor in tissue that is regarded as a normal control for such experiments. Without such essential information, much time, effort and money can be wasted and conclusions can be meaningless. The goal of the Histology and Immunohistochemistry Shared Resource is to provide these facilities to peer-reviewed Cancer Center Members and affiliated investigators. The Resource (formerly known as the Tissue Bank) has grown, during the last four years, into a widely used recharge facility and has been strengthened with additional staff. Its mission for the next funding cycle is to liaise closely with other UCSD NCI-funded Shared Resources, especially Animal Experimentation, Informatics, Molecular Pathology and Transgenic Mouse to create a superbly interactive group for the analysis of samples of human tissue, experimental animals carrying tumors, experimental animals carrying transgenes and "knock-out" mice.