CORE--HISTOLOGY AND IMMUNOHISTOCHEMISTRY

在加州大学圣地亚哥分校，许多研究人员现在正在进行研究，这需要对人类和小鼠肿瘤及其同源正常组织进行显微镜分析。其他人正在研究通过遗传和表观遗传干预在胚胎和成年动物中诱导的变化的细节，以了解调节正常和肿瘤发育的机制。这些研究人员正在研究与癌症相关的主题，他们需要随时准备好、负担得起的高质量组织学和免疫组织化学技术服务以及专家组织病理学建议。这一资源已经为该中心的成员提供了至关重要的服务，随着人们越来越广泛地认识到组织的微观评估在癌症研究中至关重要，它的重要性将会增加。这项核心服务在生化或分子生物学分析开始之前，对活检样本中肿瘤组织的存在提供至关重要的质量控制。确定组织中没有肿瘤被认为是此类实验的正常对照也得到了证实。此外，通过利用这一核心设施的服务，从胚胎阶段到成年，对转基因小鼠的组织进行完整的组织学(表型)分析是可能的。利用这些服务还可以对人类癌症的众多老鼠模型进行详细分析。组织学共享资源为所有同行评审的癌症中心成员提供高质量的组织学和免疫组织化学服务。该中心亦与其他由加州大学圣地亚哥分校NCI资助的癌症中心共享资源，特别是分子病理学、转基因小鼠和数码成像，建立一个共享资源的互动小组，以研究来自人类病理样本、人类病理样本、携带人类异种移植的实验动物，以及经基因改变的动物(转基因或“敲除”小鼠)的组织样本。

At UCSD many investigators are now conducting research which requires the microscopical analysis of human and murine tumors and their cognate normal tissues. Others are examining the details of changes induced in embryonic and adult animals by genetic and epigenetic intervention, so as to understand the mechanisms regulating normal and neoplastic development. These investigators who are studying topics relevant to cancer need ready, affordable access to high quality histological and immunohistochemical technical services and to expert histopathological advice. This resource already provides pivotally important services to the Members of the Center and its importance will grow as it becomes more widely appreciated that microscopic evaluation of tissue is critical in cancer research.. This core services provides vital quality control of the presence of tumor tissue in a biopsy sample before biochemical or molecular biological analysis begins. Establishing the absence of tumor in tissue that is regarded as a normal control for such experiments is also confirmed. In addition, by utilizing the services of this core facility, a complete histologic (phenotypic) analysis of tissues from genetically altered mice, is made possible, from the embryo stage to the adult. Utilization of the services also allows a detailed analysis of the numerous mouse models of human cancer. The Histology Shared Resources provides high quality histology and immunohistochemistry services to all peer-reviewed Cancer Center members. It also liaises closely with other UCSD NCI-funded Cancer Center Shared Resources, especially Molecular Pathology, Transgenic Mouse and Digital Imaging, to create an interactive group of shared resources for research of tissue samples from human pathological specimens, from human pathological specimens, experimental animals carrying human xenotransplants, and genetically altered animals (Transgenic or "knock-out" mice).