CORE--FLOW CYTOMETRY AND CELL SORTING

核心——流式细胞术和细胞分选

• 批准号: 6300084
• 负责人: JONNI S. MOORE
• 金额: $ 27.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-04 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300084
• 关键词: biomedical equipment resource; biomedical facility; cell population study; cellular oncology; flow cytometry

The Flow Cytometry and Cell Sorting Facility was established by the Cancer Center in 1980. It has been continuously approved and funded by the NCI Core Support Grant since 1981. Jonni Moore, PhD, has directed the Flow Cytometry and Cell Sorting Facility since 1990. Dr. Moore has a doctorate in immunology and over fifteen years of flow cytometry experience, and serves as an expert consultant to manufacturers of flow cytometry equipment (i.e., Becton Dickinson) and to other flow cytometry laboratories (e.g., SmithKline Beecham, Merck). Charles Pletcher, MCI, Technical Director has been with the Facility since 1987. The Facility has grown tremendously during the current project period. This has resulted from major operational improvements, during acquisition of newer technology, and recruitment of expert staff. With the equipment purchased during the current project period (FACSVantage and FACSCalibur), it now possesses capabilities equal to those of the most sophisticated flow cytometry facilities. Overall usage of the core has increased 67% during the current project period. Usage by Cancer Center members with peer-reviewed funding is 77% and usage by all Cancer Center members is 86% of total facility usage.

流式细胞术和细胞分选设施由癌症中心于 1980 年建立。自 1981 年以来，它不断获得 NCI 核心支持拨款的批准和资助。Jonni Moore 博士自 1990 年起负责流式细胞术和细胞分选设施。Moore 博士拥有免疫学博士学位和超过 15 年的流式细胞术经验，并担任流式细胞术制造商的专家顾问 设备（即 Becton Dickinson）和其他流式细胞术实验室（例如 SmithKline Beecham、Merck）。 Charles Pletcher，MCI，技术总监自 1987 年以来一直在该设施工作。在当前的项目期间，该设施取得了巨大的发展。这是由于采购新技术和招聘专家人员期间重大运营改进的结果。凭借在当前项目期间购买的设备（FACSVantage 和 FACSCalibur），它现在拥有与最先进的流式细胞仪设施相同的功能。在当前项目期间，核心的总体使用率增加了 67%。经同行评审资金的癌症中心成员的使用率为 77%，所有癌症中心成员的使用率为设施总使用量的 86%。