Becton Dickinson LSR 2 Flow Cytometer

Becton Dickinson LSR 2 流式细胞仪

• 批准号: 6735912
• 负责人: JONNI S. MOORE
• 金额: $ 27.3万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6735912
• 关键词: biomedical equipment purchase; calcium flux; flow cytometry; fluorescence resonance energy transfer

DESCRIPTION (provided by applicant): Researchers at the University of Pennsylvania are increasingly engaged in investigations that require greater than six-parameter flow cytometry. In addition, many investigators are making use of fluorescent resonance energy transfer (FRET) experiments to map receptor interactions. Currently, these types of multiparameter studies must be performed on the cell sorters (only 1 capable of measuring FRET with the preferred CFP/YFP pair) as no analytical instrument capable of performing sophisticated multiparameter analyses is available. Since these instruments are also used for preparative cell sorting, the wait for analytical only applications is often weeks. Lack of access to high-speed 6+ parameter analytical flow cytometry has proven to be a serious limitation to several well-funded investigators. The Becton- Dickinson LSR II (BD LSRII) is an air-cooled four-laser bench top cytometer capable of measuring more than 12 parameters simultaneously. Using a new digital acquisition system, it has enhanced detection and resolution capabilities. As configured with UV and violet lasers, it will support both calcium flux (with Indo-1) and FRET experiments, which are now available on a very limited basis. These expanded analytical capabilities to the flow cytometry core laboratory will specifically benefit the user group and many researchers in the institution and be a significant addition to the available instrumentation in the School of Medicine. In this proposal, we present a model for shared resource operation that will support the efficient use of the BD LSR II for predominantly the user group. Already in place are significant institutional support, a highly experienced staff, and a well-respected training program in flow cytometry. This will allow investigators immediate access to the service and further extend access by training selected students and technical staff to use. This will result in increased availability of high-level analytical cytometry to the scientific community. By placing the LSR II in existing recently renovated space and utilizing a well-established model for core flow cytometry facility operation, we propose to maximize the benefit of this cytometer to the user group of 17 NIH-funded investigators ($9,007,559 direct costs/year). This will allow investigators to pursue many areas of research, previously with limited availability, which will result in increased funding and advancement of biomedical science.

描述（由申请人提供）：宾夕法尼亚大学的研究人员越来越多地参与需要超过六参数流式细胞术的研究。此外，许多研究人员正在利用荧光共振能量转移（FRET）实验来绘制受体相互作用。目前，这些类型的多参数研究必须在细胞分选仪上进行（只有1个能够用优选的CFP/YFP对测量FRET），因为没有能够进行复杂的多参数分析的分析仪器可用。由于这些仪器也用于制备细胞分选，因此仅用于分析的等待时间通常为数周。缺乏高速6+参数分析流式细胞术已被证明是几个资金充足的研究人员的严重限制。 Becton-Dickinson LSR II（BD LSRII）是一款风冷式四激光台式流式细胞仪，能够同时测量12个以上的参数。它采用新的数字采集系统，提高了探测和分辨能力。由于配置了紫外和紫色激光器，它将支持钙通量（使用Indo-1）和FRET实验，这些实验现在在非常有限的基础上可用。这些扩展到流式细胞术核心实验室的分析能力将特别有利于用户组和该机构的许多研究人员，并成为医学院现有仪器的重要补充。在这个建议中，我们提出了一个共享资源操作的模型，将支持主要用户组的BD LSR II的有效使用。已经到位的是重要的机构支持，一个经验丰富的工作人员，并在流式细胞术备受推崇的培训计划。这将使调查人员能够立即使用该服务，并通过培训选定的学生和技术人员来进一步扩大使用范围。这将使科学界更容易获得高水平的分析细胞仪。 通过将LSR II放置在现有的最近翻新的空间，并利用一个成熟的模型，核心流式细胞仪设施的操作，我们建议最大限度地提高这台细胞仪的好处，17个NIH资助的研究人员的用户组（9,007,559美元直接成本/年）。这将使研究人员能够从事以前有限的研究领域，这将导致资金增加和生物医学科学的进步。