METABOLIC EFFECT OF TRP64ARG MUTATION IN BETA 3 ADRENERGIC RECEPTORS IN DIABETES

贝勒医学院和人类学系的研究人员 公共卫生学院，健康科学中心， 休斯顿，德克萨斯州将研究β肾上腺素能受体的影响， 激活对西班牙裔男性和女性的脂质和葡萄糖代谢的影响 非胰岛素依赖型糖尿病（NIDDM）。 的目标 这些研究是为了确定是否有一个可预测的 β 3-肾上腺素能受体（AR）突变、机体 墨西哥裔美国人的脂肪分布和脂质及葡萄糖代谢。 通过使用营养和运动作为实验操作，我们计划 为了确定这种突变在调节燃料中的作用， 健康人基质代谢及其在NIDDM中的失调科目 将在哈里斯县医院的病人中进行鉴定 东南部和南部的地区和斯塔尔县健康研究 德克萨斯它们将根据基因组DNA中的模式进行表征 从白细胞、身体组成和腹部脂肪的分布 使用MRI，测量血糖和胰岛素的血浆水平 空腹和口服葡萄糖后。 约850 受试者将在横断面研究中进行表征， 这些课题将使用稳定同位素进行更深入的研究， 基线、进食、空腹、休息和运动下的技术 条件 这些研究在24名受试者中进行， 一个月的逐步减肥，目的是减少身体 体重增加5 - 10公斤，减少腹内脂肪5%-15%。

Investigators in Baylor College of Medicine and the Department of Human Genetics at the School of Public Health, Health Sciences Center, Houston, TX will study the effects of beta adrenergic receptor activation on lipid and glucose metabolism in Hispanic men and women with non-insulin-dependent diabetes mellitus (NIDDM). The objective of these studies is to determine whether there is a predictable relationship between the beta 3-adrenergic receptor (AR) mutation, body fat distribution and lipid and glucose metabolism in Mexican-Americans. By using nutrition and exercise as experimental manipulations, we plan to determine the role of this mutation in the regulation of fuel substrate metabolism in health and its dysregulation in NIDDM. Subjects will be identified among patients served in the Harris County Hospital District and the Starr County Health Studies in Southeast and South Texas. They will be characterized according to patterns in genomic DNA from leukocytes, body composition and distribution of abdominal fat using MRI, and plasma levels of glucose and insulin will be measured during fasting and after oral glucose administration. Approximately 850 subjects will be characterized in the cross-sectional study and 24 of these subjects will be studied more intensively using stable isotopic techniques under baseline, fed, fasting, resting, and exercising conditions. These studies in the 24 subjects will be repeated after one month of gradual weight reduction with the objective of decreasing body weight by 5 to 10 kg and reducing intraabdominal fat by 5% to 15%.