Flow Cytometric Method to Analyze Micronuclei

流式细胞仪分析微核的方法

• 批准号: 6403333
• 负责人: DOROTHEA K TOROUS
• 金额: $ 26.55万
• 依托单位: LITRON LABORATORIES, LTD.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6403333
• 关键词: DNA damage; cell component structure /function; flow cytometry; laboratory mouse; micronucleus; microscopy; peripheral blood vessel; technology /technique development; tissue /cell preparation

DESCRIPTION: The in vivo micronucleus (MN) assay is a widely used test to screen compounds for chromosome-damaging activity. It is also a key test for the registration of new pharmaceuticals. Litron Laboratories has developed an automated method of scoring MN using flow cytometry (FCM). This system has several benefits: increased numbers of cells analyzed, ability to process more samples in less time, and objective analysis/scoring. In Phase II work Litron will perform experiments designed to validate their kit for scoring MN. Specifically, they and their collaborators will: analyze genotoxic compounds with different modes of action, perform the entire assay from animal dosing to blood fixation and analysis, perform microscopic evaluations of these same blood samples for comparison with FCM, measure interlaboratory variability, and prepare a document to submit to ICCVAM for regulatory approval. PROPOSED COMMERCIAL APPLICATION: The validation and regulatory approval of Litron's FCM based MN assay would result in increased sales of Litron's developed mouse peripheral blood MN analysis kits. Similar studies are planned for analysis kits to measure MN in mouse and rat bone marrow, rat peripheral blood and also human peripheral blood. Enormous commercial opportunity exists from the sale of these kits.

体内微核（MN）测定是一种广泛使用的检测方法